BS EN ISO 6579-4:2025 PDF

Microbiology of the food chain. Horizontal method for the detection, enumeration and serotyping of Salmonella - Identification of monophasic Salmonella Typhimurium (1,4,[5],12:i:-) by polymerase chain reaction (PCR)

First page of standard BS EN ISO 6579-4:2025

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Title (English):

BS EN ISO 6579-4:2025

Document status:: Active
Format:: Electronic (PDF)
Publication date:: June 30, 2025
ICS:: 07.100.30
Technical committee:: CEN
SKU:: BS EN ISO 6579-4:2025

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Abstract

1 Scope This document specifies a horizontal in vitro method for the molecular identification and differentiation of the monophasic variant of Salmonella enterica subsp. enterica serovar Typhimurium ( 1 ,4,[5],12:i:-) lacking the second H phase H:1,2, starting from isolates. The method detects specific DNA sequences of an intergenic region of the first H phase flagellin cluster for identification of Salmonella enterica subsp. enterica serovar Typhimurium (further called Salmonella Typhimurium) and specific DNA sequences of genes associated with second H phase flagellar antigen expression. The method is applicable for:

— differentiation of the isolate under analysis between monophasic Salmonella Typhimurium and the monophasic variant of another Salmonella non-Typhimurium serovar that has the same antigenic formula;

— identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium.

This document is applicable for the analysis of a pure culture belonging to the genus Salmonella , isolated from:

— products intended for human consumption;

...

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Other BS standards

— identification of the isolate under analysis being either monophasic Salmonella Typhimurium or (biphasic) Salmonella Typhimurium.

This document is applicable for the analysis of a pure culture belonging to the genus Salmonella , isolated from:

— products intended for human consumption;

...

BS EN ISO 6579-4:2025 PDF

Abstract

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Abstract

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