EN ISO 18363-1:2021 PDF

Animal and vegetable fats and oils - Determination of fatty-acid-bound chloropropanediols (MCPDs) and glycidol by GC/MS - Part 1: Method using fast alkaline transesterification and measurement for 3-MCPD and differential measurement for glycidol (ISO 18363-1:2015)

Title (English):

EN ISO 18363-1:2021

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 20
Publication date:: December 15, 2021
Edition:: CEN EN 18363 edition 1 version 1
ICS:: 67.200.10

Document language:

enru

Delivery time:1–7 days

Price:66 €

Description (English):

ISO 18363-1:2015 describes a procedure for the indirect determination of 3-MCPD esters (bound 3-MCPD) and possible free 3-MCPD after alkaline catalysed ester cleavage and derivatization with phenylboronic acid (PBA). Furthermore, this part of ISO 18363 enables the indirect determination of glycidyl esters (bound glycidol) under the assumption that no other substances are present that react at room temperature with inorganic chloride to generate 3-MCPD. ISO 18363-1:2015 is applicable to solid and liquid fats and oils. Milk and milk products (or fat coming from milk and milk products) are excluded from the scope of this part of ISO 18363.

Technical details

Technical committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
SKU: EN ISO 18363-1:2021

Abstract

Overview

EN ISO 18363-1:2021 (ISO 18363-1:2015) specifies a GC/MS method for the indirect determination of fatty-acid‑bound chloropropanediols (MCPDs) and glycidol in animal and vegetable fats and oils. The method uses a fast alkaline transesterification, followed by derivatization (phenylboronic acid, PBA) and gas chromatography–mass spectrometry (GC/MS) analysis. It enables measurement of 3‑MCPD esters and a differential determination of glycidol (by converting glycidol to induced 3‑MCPD under controlled conditions). Milk and milk‑derived fats are excluded from the scope. The standard is published by CEN as EN ISO 18363‑1:2021 and is identical to ISO 18363‑1:2015.

Key topics and technical requirements

Analytes covered: ester‑bound 3‑MCPD and glycidyl esters (glycidol), including possible free 3‑MCPD present in samples.
Analytical principle: fast alkaline catalysed alcoholysis at room temperature to release bound analytes, PBA derivatization and GC/MS detection.
Differential approach: two assays (A and B) -
- Part A converts glycidol to 3‑MCPD using an acidified chloride solution so 3‑MCPD + induced 3‑MCPD are measured together.
- Part B uses a chloride‑free stop solution to measure genuine 3‑MCPD only. Glycidol concentration is calculated from the difference (A − B) using a determined transformation ratio.
Scope limits & assumptions: Applicable to solid and liquid fats and oils (refined and non‑refined); excludes milk/milk products. Indirect glycidyl ester determination assumes no other sample components react with chloride at room temperature to produce 3‑MCPD.
Method structure: reagent specifications, apparatus, sampling/preparation, spiking with surrogate standards, ester cleavage, matrix cleanup, derivatization, GC/MS conditions, expression of results, and precision (interlaboratory, repeatability, reproducibility).

Applications and users

Food safety and regulatory testing: routine monitoring of 3‑MCPD and glycidol in edible oils and fats to meet regulatory limits and risk assessments.
Industrial quality control: refining plants, oil processors and frying‑oil users monitoring process steps that can generate MCPDs/glycidol.
Analytical and research laboratories: method implementation for surveillance, method validation, and interlaboratory studies.
Who should use it: QC chemists, food control authorities, contract testing labs, and researchers working on contaminants in fats and oils.

Related standards

EN ISO 18363 series (other parts cover alternative transesterification and measurement approaches).
The method is equivalent to DGF standard C‑VI 18 (10) and identical to AOCS Official Method Cd 29c‑13 (as noted in the ISO introduction).

Keywords: EN ISO 18363-1:2021, ISO 18363-1:2015, 3‑MCPD, glycidol, glycidyl esters, MCPD esters, GC/MS, alkaline transesterification, animal and vegetable fats and oils, food safety testing.

Related standards

Mentioned in the description and other EN standards

Abstract

Overview

Key topics and technical requirements

Analytes covered: ester‑bound 3‑MCPD and glycidyl esters (glycidol), including possible free 3‑MCPD present in samples.
Analytical principle: fast alkaline catalysed alcoholysis at room temperature to release bound analytes, PBA derivatization and GC/MS detection.
Differential approach: two assays (A and B) -
- Part A converts glycidol to 3‑MCPD using an acidified chloride solution so 3‑MCPD + induced 3‑MCPD are measured together.
- Part B uses a chloride‑free stop solution to measure genuine 3‑MCPD only. Glycidol concentration is calculated from the difference (A − B) using a determined transformation ratio.
Scope limits & assumptions: Applicable to solid and liquid fats and oils (refined and non‑refined); excludes milk/milk products. Indirect glycidyl ester determination assumes no other sample components react with chloride at room temperature to produce 3‑MCPD.
Method structure: reagent specifications, apparatus, sampling/preparation, spiking with surrogate standards, ester cleavage, matrix cleanup, derivatization, GC/MS conditions, expression of results, and precision (interlaboratory, repeatability, reproducibility).

Applications and users

Food safety and regulatory testing: routine monitoring of 3‑MCPD and glycidol in edible oils and fats to meet regulatory limits and risk assessments.
Industrial quality control: refining plants, oil processors and frying‑oil users monitoring process steps that can generate MCPDs/glycidol.
Analytical and research laboratories: method implementation for surveillance, method validation, and interlaboratory studies.
Who should use it: QC chemists, food control authorities, contract testing labs, and researchers working on contaminants in fats and oils.

Related standards

EN ISO 18363 series (other parts cover alternative transesterification and measurement approaches).
The method is equivalent to DGF standard C‑VI 18 (10) and identical to AOCS Official Method Cd 29c‑13 (as noted in the ISO introduction).

Keywords: EN ISO 18363-1:2021, ISO 18363-1:2015, 3‑MCPD, glycidol, glycidyl esters, MCPD esters, GC/MS, alkaline transesterification, animal and vegetable fats and oils, food safety testing.

EN ISO 18363-1:2021 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Related standards

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Related standards

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