EN ISO 6887-6:2013 PDF

Microbiology of food and animal feed - Preparation of test samples, initial suspension and decimal dilutions for microbiological examination - Part 6: Specific rules for the preparation of samples taken at the primary production stage (ISO 6887-6:2013)

Title (English):

EN ISO 6887-6:2013

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 16
Publication date:: March 6, 2013
Edition:: CEN EN 6887 edition 1 version 1
ICS:: 07.100.30

Document language:

enru

Delivery time:1–7 days

Price:60 €

Description (English):

ISO 6887-6:2013 specifies rules for the preparation of samples taken at all stages from the farm to the slaughterhouse and their suspension for microbiological examination when the samples require different preparation from the methods described in ISO 6887-1. ISO 6887-6:2013 excludes the preparation of samples for both enumeration and detection test methods where preparation details are specified in the relevant International Standards. ISO 6887-6:2013 is applicable to various samples taken from the hatchery, the farm, from the vehicle or the animals during transportation, or from animals or their carcasses in the slaughterhouse, to indicate the microbiological status of the animals in relation to zoonotic agents. ISO 6887-6:2013 does not apply to samples taken to assess the hygiene of meat.

Technical details

Technical committee: CEN/TC 463 - Microbiology of the food chain
SKU: EN ISO 6887-6:2013

Abstract

Overview

EN ISO 6887-6:2013 (ISO 6887-6:2013) sets specific rules for preparing samples taken at the primary production stage for microbiological examination. It complements ISO 6887-1 (general rules) by addressing samples collected from the hatchery, farm, vehicles or animals during transport, and from animals or carcasses at the slaughterhouse when these require different preparation. The standard helps determine the microbiological status of animals with respect to zoonotic agents. It excludes preparations already specified in other standards and does not cover meat-hygiene sampling or aquatic primary production (covered by other parts of ISO 6887).

Key topics and technical requirements

Scope and exclusions: Applies to primary production-stage samples (farm → slaughterhouse) but excludes sample preparations already detailed in other International Standards and hygiene samples for meat (ISO 6887-2) or aquatic samples (ISO 6887-3).
Principle: Preparation of an initial suspension (commonly 1 in 10, i.e. a nine-fold diluent) to obtain a uniform microbial distribution without reducing viability; preparation of further decimal dilutions for enumeration or detection.
Terms and definitions: Laboratory sample, test portion, pooled sample, initial suspension, further decimal dilutions-standardizes language for laboratory transfer and testing.
Diluents and neutralizers: Use of standard diluents per ISO 6887-1; neutralizing liquid (ISO 13307) can be included (typically 10 % v/v) and agents such as L‑histidine may be recommended if formalin is expected.
Apparatus and consumables: Homogenizers (stomacher, blender, pulsifier), sterile tools (forceps, scalpels), pipettes, screw-cap bottles, sterile sand/pestle when needed.
Sample types & handling: Guidance on laboratory samples, pooled samples, storage and transport per ISO 7218 and ISO 13307; differentiation of procedures for samples taken on-farm, at slaughterhouse, or from poultry during hatchery/transport.

Applications and users

Who uses it: Food and feed microbiology laboratories, veterinary diagnostic labs, food safety authorities, primary-producer quality teams, slaughterhouse QA personnel, and microbiologists conducting zoonotic-agent surveillance.
Practical uses:
- Preparing representative test portions and suspensions for microbiological testing of animals and carcasses.
- Standardizing pre-enrichment and enrichment suspensions for detection methods (unless another standard specifies preparation).
- Supporting surveillance of zoonotic agents from hatchery to slaughterhouse.
- Ensuring consistent dilution series for reliable enumeration and reporting.

Related standards

ISO 6887-1 - General rules for initial suspension and decimal dilutions
ISO 6887-2 - Meat and meat products (hygiene sampling)
ISO 6887-3, -4, -5 - Other product-specific preparation rules
ISO 7218 - General requirements and guidance for microbiological examinations
ISO 13307 - Sampling techniques at primary production stage

Keywords: ISO 6887-6, microbiology of food, primary production, sample preparation, initial suspension, decimal dilutions, zoonotic agents, food microbiology.

Related standards

Standards mentioned in the description

Abstract

Overview

Key topics and technical requirements

Scope and exclusions: Applies to primary production-stage samples (farm → slaughterhouse) but excludes sample preparations already detailed in other International Standards and hygiene samples for meat (ISO 6887-2) or aquatic samples (ISO 6887-3).
Principle: Preparation of an initial suspension (commonly 1 in 10, i.e. a nine-fold diluent) to obtain a uniform microbial distribution without reducing viability; preparation of further decimal dilutions for enumeration or detection.
Terms and definitions: Laboratory sample, test portion, pooled sample, initial suspension, further decimal dilutions-standardizes language for laboratory transfer and testing.
Diluents and neutralizers: Use of standard diluents per ISO 6887-1; neutralizing liquid (ISO 13307) can be included (typically 10 % v/v) and agents such as L‑histidine may be recommended if formalin is expected.
Apparatus and consumables: Homogenizers (stomacher, blender, pulsifier), sterile tools (forceps, scalpels), pipettes, screw-cap bottles, sterile sand/pestle when needed.
Sample types & handling: Guidance on laboratory samples, pooled samples, storage and transport per ISO 7218 and ISO 13307; differentiation of procedures for samples taken on-farm, at slaughterhouse, or from poultry during hatchery/transport.

Applications and users

Who uses it: Food and feed microbiology laboratories, veterinary diagnostic labs, food safety authorities, primary-producer quality teams, slaughterhouse QA personnel, and microbiologists conducting zoonotic-agent surveillance.
Practical uses:
- Preparing representative test portions and suspensions for microbiological testing of animals and carcasses.
- Standardizing pre-enrichment and enrichment suspensions for detection methods (unless another standard specifies preparation).
- Supporting surveillance of zoonotic agents from hatchery to slaughterhouse.
- Ensuring consistent dilution series for reliable enumeration and reporting.

Related standards

ISO 6887-1 - General rules for initial suspension and decimal dilutions
ISO 6887-2 - Meat and meat products (hygiene sampling)
ISO 6887-3, -4, -5 - Other product-specific preparation rules
ISO 7218 - General requirements and guidance for microbiological examinations
ISO 13307 - Sampling techniques at primary production stage

Keywords: ISO 6887-6, microbiology of food, primary production, sample preparation, initial suspension, decimal dilutions, zoonotic agents, food microbiology.

EN ISO 6887-6:2013 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Related standards

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Related standards

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