EN ISO 8692:2012 PDF

Water quality - Fresh water algal growth inhibition test with unicellular green algae (ISO 8692:2012)

Title (English):

EN ISO 8692:2012

Water quality - Fresh water algal growth inhibition test with unicellular green algae (ISO 8692:2012)

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 28
Publication date:: February 15, 2012
Edition:: CEN EN 8692 edition 2 version 1
ICS:: 13.060.70

Document language:

enru

Delivery time:1–7 days

Price:82 €

Description (English):

ISO 8692:2012 specifies a method for the determination of the growth inhibition of unicellular green algae by substances and mixtures contained in water or by waste water. This method is applicable for substances that are easily soluble in water. With modifications to this method, as specified in ISO 14442 and ISO 5667-16, the inhibitory effects of poorly soluble organic and inorganic materials, volatile compounds, heavy metals and waste water can be tested. A rapid algal growth inhibition screening test for waste water is described in an annex. An alternative test procedure with algae from algal beads, with direct measurement of algal growth in spectrophotometric cells, is described in an annex.

Technical details

Technical committee: CEN/TC 230 - Water analysis
SKU: EN ISO 8692:2012

Abstract

Overview - EN ISO 8692:2012 (ISO 8692:2012)

EN ISO 8692:2012 specifies a standardized laboratory method for assessing the growth inhibition of unicellular green algae in freshwater caused by substances, mixtures or waste water. Designed for water quality and ecotoxicity testing, the method measures reductions in algal growth over a 72 ± 2 h exposure and derives effect concentrations (e.g., EC50, other ECx values) based on specific growth rate comparisons to control cultures.

Key topics and requirements

Test organisms: Recommended planktonic species include Desmodesmus subspicatus and Pseudokirchneriella subcapitata (specific culture strains cited).
Test principle: Monospecies cultures are exposed to a concentration series; cell density is measured at least every 24 h; inhibition is expressed as reduced specific growth rate relative to controls.
Duration and sampling: Standard incubation period is 72 ± 2 hours, with periodic density measurements (minimum every 24 h).
Applicability: Primarily for water-soluble substances; with modifications (referenced standards) the method can handle poorly soluble materials, volatile compounds, heavy metals and waste water.
Annex procedures:
- Annex A - rapid algal growth inhibition screening for waste water.
- Annex B - alternative procedure using algal beads with direct spectrophotometric measurement.
- Annex C - protocol for immobilizing algae in alginate beads.
Quality controls: Requirements include defined growth media, reagent purity, deionized water (conductivity

Related standards

Mentioned in the description and other EN standards

Overview - EN ISO 8692:2012 (ISO 8692:2012)

Key topics and requirements

Test organisms: Recommended planktonic species include Desmodesmus subspicatus and Pseudokirchneriella subcapitata (specific culture strains cited).
Test principle: Monospecies cultures are exposed to a concentration series; cell density is measured at least every 24 h; inhibition is expressed as reduced specific growth rate relative to controls.
Duration and sampling: Standard incubation period is 72 ± 2 hours, with periodic density measurements (minimum every 24 h).
Applicability: Primarily for water-soluble substances; with modifications (referenced standards) the method can handle poorly soluble materials, volatile compounds, heavy metals and waste water.
Annex procedures:
- Annex A - rapid algal growth inhibition screening for waste water.
- Annex B - alternative procedure using algal beads with direct spectrophotometric measurement.
- Annex C - protocol for immobilizing algae in alginate beads.
Quality controls: Requirements include defined growth media, reagent purity, deionized water (conductivity

EN ISO 8692:2012 PDF

Technical details

Abstract

Overview - EN ISO 8692:2012 (ISO 8692:2012)

Key topics and requirements

Related standards

Abstract

Overview - EN ISO 8692:2012 (ISO 8692:2012)

Key topics and requirements

Related standards

Water quality — Fresh water algal growth inhibition test with unicellular green algae

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