ISO 11348-3:2007 PDF

Water quality — Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) — Part 3: Method using freeze-dried bacteria

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Title (English):

ISO 11348-3:2007

Water quality — Determination of the inhibitory effect of water samples on the light emission of Vibrio fischeri (Luminescent bacteria test) — Part 3: Method using freeze-dried bacteria

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 21
Publication date:: November 27, 2007
Edition:: ISO IS 11348 edition 2 version 1
ICS:: 13.060.70

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Description (English):

ISO 11348 describes three methods for determining the inhibition of the luminescence emitted by the marine bacterium Vibrio fischeri (NRRL B‑11177). ISO 11348-3:2007 specifies a method using freeze‑dried bacteria. This method is applicable to: waste water; aqueous extracts and leachates; fresh water (surface and ground water); sea and brackish water; eluates of sediment (freshwater, brackish and sea water); pore water; single substances, diluted in water.

Technical details

Technical committee: ISO/TC 147/SC 5 - Biological methods
SKU: ISO 11348-3:2007

Abstract

Overview

ISO 11348-3:2007 specifies a standardized luminescent bacteria test using freeze‑dried Vibrio fischeri (NRRL B‑11177) to assess the inhibitory effect of water samples on bacterial light emission. It is one part of the ISO 11348 series for measuring toxicity by luminescence inhibition. The method applies to wastewater, surface and groundwater, sea and brackish water, sediment eluates, pore water, leachates, and single substances diluted in water.

Key topics and technical requirements

Test organism & reagents: Uses commercially available freeze‑dried Vibrio fischeri reconstituted with a specified salt solution (20 g/L NaCl; 2.035 g/L MgCl2·6H2O; 0.30 g/L KCl). Store freeze‑dried bacteria at −18 °C to −20 °C.
Diluent & reference substances: Sodium chloride diluent (20 g/L NaCl). Reference reagents include zinc sulfate, 3,5‑dichlorophenol and potassium dichromate prepared as stock solutions for quality control.
Apparatus & conditions: Luminometer with thermostatted measuring cell (15 °C ±1 °C), thermo‑block, pH meter, oxygen probe (ISO 5814), accurate pipettes and freezer/incubator.
Sample preparation: Measure oxygen (>3 mg/L required), adjust pH when necessary (acceptable test range pH 6.0–8.5), manage salinity (add NaCl to 20 g/L where appropriate; avoid hyperosmotic test conditions above ~35 g/L NaCl equivalent), reduce turbidity by settling/centrifugation/filtration.
Procedure & evaluation: Batch test combining sample (or dilution series) with bacterial suspension; luminescence measured after defined contact times (5, 15 or 30 min). Results expressed as percent inhibition, LID (see Annex B) and/or EC values (e.g., EC20, EC50) derived from dilution series.
Interferences & validity: Colour, turbidity, oxygen demand, pH extremes, and high salinity can bias results; the standard includes correction methods (Annex A) and validity/precision criteria (Annex C).

Applications and users

ISO 11348-3 is used for rapid toxicity screening and comparative biotesting in:

Environmental and public health laboratories
Wastewater and industrial effluent monitoring
Regulatory compliance and permitting programs
Research on aquatic toxicity, sediment eluates and leachate impact
Contract environmental testing and consulting

This method helps detect toxic effects from metals, organics and complex mixtures and provides quantifiable endpoints (EC values) for risk assessment and treatment evaluation.

Related standards

ISO 11348-1: fresh bacterial cultures method
ISO 11348-2: liquid‑dried bacteria method
ISO 5667-16: sampling guidance for biotesting
ISO 5814: dissolved oxygen measurement

Keywords: ISO 11348-3, luminescent bacteria test, Vibrio fischeri, freeze-dried bacteria, water quality testing, toxicity testing, EC50, biotesting.

Related standards

Standards mentioned in the description

Abstract

Overview

Key topics and technical requirements

Test organism & reagents: Uses commercially available freeze‑dried Vibrio fischeri reconstituted with a specified salt solution (20 g/L NaCl; 2.035 g/L MgCl2·6H2O; 0.30 g/L KCl). Store freeze‑dried bacteria at −18 °C to −20 °C.
Diluent & reference substances: Sodium chloride diluent (20 g/L NaCl). Reference reagents include zinc sulfate, 3,5‑dichlorophenol and potassium dichromate prepared as stock solutions for quality control.
Apparatus & conditions: Luminometer with thermostatted measuring cell (15 °C ±1 °C), thermo‑block, pH meter, oxygen probe (ISO 5814), accurate pipettes and freezer/incubator.
Sample preparation: Measure oxygen (>3 mg/L required), adjust pH when necessary (acceptable test range pH 6.0–8.5), manage salinity (add NaCl to 20 g/L where appropriate; avoid hyperosmotic test conditions above ~35 g/L NaCl equivalent), reduce turbidity by settling/centrifugation/filtration.
Procedure & evaluation: Batch test combining sample (or dilution series) with bacterial suspension; luminescence measured after defined contact times (5, 15 or 30 min). Results expressed as percent inhibition, LID (see Annex B) and/or EC values (e.g., EC20, EC50) derived from dilution series.
Interferences & validity: Colour, turbidity, oxygen demand, pH extremes, and high salinity can bias results; the standard includes correction methods (Annex A) and validity/precision criteria (Annex C).

Applications and users

ISO 11348-3 is used for rapid toxicity screening and comparative biotesting in:

Environmental and public health laboratories
Wastewater and industrial effluent monitoring
Regulatory compliance and permitting programs
Research on aquatic toxicity, sediment eluates and leachate impact
Contract environmental testing and consulting

This method helps detect toxic effects from metals, organics and complex mixtures and provides quantifiable endpoints (EC values) for risk assessment and treatment evaluation.

Related standards

ISO 11348-1: fresh bacterial cultures method
ISO 11348-2: liquid‑dried bacteria method
ISO 5667-16: sampling guidance for biotesting
ISO 5814: dissolved oxygen measurement

Keywords: ISO 11348-3, luminescent bacteria test, Vibrio fischeri, freeze-dried bacteria, water quality testing, toxicity testing, EC50, biotesting.

ISO 11348-3:2007 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Related standards

Abstract

Overview

Key topics and technical requirements

Applications and users

Related standards

Related standards

Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fisch…

Water quality - Determination of dissolved oxygen - Electrochemical probe method (ISO 5814:2012)

Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fisch…

Water quality - Determination of the inhibitory effect of water samples on the light emission of Vibrio fisch…

Water quality - Sampling - Part 16: Guidance on biotesting of samples (ISO 5667-16:2017)