Overview
ISO 13144:2025 specifies a laboratory method for the qualification and quantification of quinoline, isoquinoline and certain quinoline derivatives in textiles. The method uses methanol extraction followed by analysis with gas chromatography–mass spectrometry (GC‑MS / GC‑MS/MS) or liquid chromatography–mass spectrometry (LC‑MS / LC‑MS/MS). It applies to natural and synthetic dyed fibres and fabrics as well as to dyestuff powders used for textile dyeing and printing.
Key topics and technical requirements
- Scope and analytes: Target compounds include quinoline, isoquinoline and selected methyl/dimethyl quinoline isomers (see Table 1 of the standard for the full list and CAS numbers).
- Extraction principle: Samples are extracted with methanol in an ultrasonic water bath (≈70 °C) to transfer analytes into solution for instrumental analysis.
- Sample preparation:
- Textile test specimen typical mass: (1.0 ± 0.1) g (minimum 0.5 g if limited material).
- Dyestuff test specimen typical mass: ≈0.1 g.
- Extraction solvent volume: 15 ml methanol; extraction time 30 ± 2 min.
- Filtration of the cooled extract (1–2 ml) through a PTFE membrane filter (≤0.45 µm) prior to analysis.
- Standards and calibration:
- Stock solutions: 1 000 µg/ml in methanol; store protected from light (brown bottle) at about 6 °C.
- Prepare at least four calibration solutions daily to establish a calibration curve.
- Apparatus and analytical systems: analytical balance (≥0.1 mg resolution), ultrasonic bath, volumetric glassware, pipettes, chromatographic vials and instruments capable of GC‑MS, GC‑MS/MS, LC‑MS or LC‑MS/MS.
- Quality and reporting: Clauses cover calibration curves, calculation of results, method reliability and limit of quantification. Informative annexes give example GC‑MS and LC‑MS methods and reliability data.
- Safety note: The procedure involves hazardous chemicals and equipment; users must follow applicable health, safety and environmental controls.
Practical applications and users
- Textile testing laboratories verifying residues of quinoline derivatives for regulatory compliance (for example, jurisdictions restricting quinoline in clothing and skin-contact textiles).
- Manufacturers and dye houses performing in‑house quality control of dyed fabrics and dyestuff powders.
- Compliance and regulatory bodies assessing conformity with chemical restrictions and consumer safety.
- Environmental and toxicology labs monitoring potential release of quinoline derivatives from textile processes.
Related standards
- ISO 3696 - Water for analytical laboratory use (referenced for water quality).
- ISO 4787 - Laboratory glass and plastic ware - Volumetric instruments.
- ISO 13144 includes informative annexes (A–C) with chromatographic examples and method reliability guidance.
