ISO 13365-2:2020 PDF

Leather — Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography — Part 2: Artificial perspiration extraction method

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Title (English):

ISO 13365-2:2020

Leather — Chemical determination of the preservative (TCMTB, PCMC, OPP, OIT) content in leather by liquid chromatography — Part 2: Artificial perspiration extraction method

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 7
Publication date:: August 3, 2020
Edition:: ISO IS 13365 edition 1 version 1
ICS:: 59.140.30

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Description (English):

This document specifies a test method by artificial perspiration solution aqueous extraction for the determination of the aqueous extractable content of the following preservative agents in leather by liquid chromatography: — 2-(thiocyanomethylthio)-benzothiazole (TCMTB); — 4-chloro-3-methylphenol (PCMC); — 2-phenylphenol (OPP); — 2-octylisothiazol-3(2H)-one (OIT); This method can also be used to determine breakdown products of these preservative agents, which protect leather from microbiological attack.

Technical details

Technical committee: IULTCS - International Union of Leather Technologists and Chemists Societies
SKU: ISO 13365-2:2020

Abstract

Overview

ISO 13365-2:2020 specifies an artificial perspiration extraction method for determining the aqueous-extractable content of leather preservatives - TCMTB, PCMC, OPP, and OIT - by liquid chromatography (HPLC/LC‑MS). The procedure assesses the portion of preservative agents that can be released from leather under simulated perspiration conditions, helping evaluate potential user exposure and product safety.

Key topics and technical requirements

Scope: Quantification of TCMTB (2-(thiocyanomethylthio)-benzothiazole), PCMC (4-chloro-3-methylphenol), OPP (2-phenylphenol) and OIT (2-octylisothiazol-3(2H)-one) and their breakdown products using aqueous extraction and liquid chromatography.
Extraction medium: Freshly prepared acid artificial perspiration solution (pH 5.5 ± 0.1) containing L-histidine·HCl, NaCl and NaH2PO4·2H2O.
Sample extraction: Typically ~1 g leather with 10 ml artificial perspiration, extracted in a thermostatic shaker at 37 ± 2 °C for 24 ± 1 h.
Analysis: Filtered extract analysed by HPLC with UV detection or LC‑MS. Calibration requires multiple concentrations and linear calibration curves.
Calculations & reporting: Mass fraction expressed in mg/kg using the standard formula (concentration × extract volume × dilution factor ÷ sample mass). Results can be reported on a dry-matter basis per ISO 4684. Test report must reference ISO 13365-2 and include detection type and any deviations.
Apparatus and reagents: Reversed-phase C8/C18 columns, HPLC or LC‑MS systems, specified reagent grades (HPLC/LC‑MS grade solvents, reference standards ≥97–99.7 %), membrane filters and controlled water quality (ISO 3696).

Practical applications

Compliance and safety testing for leather products (footwear, garments, upholstery) to assess preservative release under simulated use.
Quality-control testing in tanneries and leather finishing operations to monitor preservative levels.
Exposure and risk assessment for product safety, regulatory submissions, and supplier verification.
Analytical method for contract testing laboratories performing routine preservative screening.

Who should use this standard

Leather technologists and chemists
Quality assurance labs in tanneries and leather product manufacturers
Third‑party testing laboratories and regulatory bodies
Product safety and compliance teams evaluating chemical release from leather

Related standards

ISO 13365-1 (other extraction method in the same series)
ISO 2418 (sampling location), ISO 4044 (sample preparation), ISO 4684 (volatile matter/dry matter), ISO 3696 (water quality)

Keywords: ISO 13365-2, leather preservative testing, artificial perspiration extraction, TCMTB, PCMC, OPP, OIT, HPLC, LC‑MS, leather safety, preservative content.

Related standards

Standards mentioned in the description

Abstract

Overview

Key topics and technical requirements

Scope: Quantification of TCMTB (2-(thiocyanomethylthio)-benzothiazole), PCMC (4-chloro-3-methylphenol), OPP (2-phenylphenol) and OIT (2-octylisothiazol-3(2H)-one) and their breakdown products using aqueous extraction and liquid chromatography.
Extraction medium: Freshly prepared acid artificial perspiration solution (pH 5.5 ± 0.1) containing L-histidine·HCl, NaCl and NaH2PO4·2H2O.
Sample extraction: Typically ~1 g leather with 10 ml artificial perspiration, extracted in a thermostatic shaker at 37 ± 2 °C for 24 ± 1 h.
Analysis: Filtered extract analysed by HPLC with UV detection or LC‑MS. Calibration requires multiple concentrations and linear calibration curves.
Calculations & reporting: Mass fraction expressed in mg/kg using the standard formula (concentration × extract volume × dilution factor ÷ sample mass). Results can be reported on a dry-matter basis per ISO 4684. Test report must reference ISO 13365-2 and include detection type and any deviations.
Apparatus and reagents: Reversed-phase C8/C18 columns, HPLC or LC‑MS systems, specified reagent grades (HPLC/LC‑MS grade solvents, reference standards ≥97–99.7 %), membrane filters and controlled water quality (ISO 3696).

Practical applications

Compliance and safety testing for leather products (footwear, garments, upholstery) to assess preservative release under simulated use.
Quality-control testing in tanneries and leather finishing operations to monitor preservative levels.
Exposure and risk assessment for product safety, regulatory submissions, and supplier verification.
Analytical method for contract testing laboratories performing routine preservative screening.

Who should use this standard

Leather technologists and chemists
Quality assurance labs in tanneries and leather product manufacturers
Third‑party testing laboratories and regulatory bodies
Product safety and compliance teams evaluating chemical release from leather

Related standards

ISO 13365-1 (other extraction method in the same series)
ISO 2418 (sampling location), ISO 4044 (sample preparation), ISO 4684 (volatile matter/dry matter), ISO 3696 (water quality)

Keywords: ISO 13365-2, leather preservative testing, artificial perspiration extraction, TCMTB, PCMC, OPP, OIT, HPLC, LC‑MS, leather safety, preservative content.

ISO 13365-2:2020 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Practical applications

Who should use this standard

Related standards

Related standards

Abstract

Overview

Key topics and technical requirements

Practical applications

Who should use this standard

Related standards

Related standards

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