Overview
ISO 16000-19:2012 - Indoor air - Part 19: Sampling strategy for moulds defines a standardized sampling strategy for detecting fungi (moulds) in indoor environments. The standard describes suitable sampling and analysis approaches, applicability and interpretation of results to maximize comparability of measured data for a given measurement objective. It focuses on measurement strategy and methods for mould detection; it does not replace building inspections, building-physics procedures or provide quantitative exposure assessment for occupants. Application of ISO 16000-19 presupposes familiarity with ISO 16000-1 (general sampling strategy).
Key topics and requirements
- Scope and purpose: Guidance to identify indoor mould sources and to standardize sampling to improve data comparability.
- Properties and occurrence: Describes origin, variability and behaviour of mould spores in indoor air (seasonal and site-dependent variability; typical spore size range).
- Sampling and detection methods: Summarizes applicable techniques (e.g., filtration, impaction, culture-based and total spore count approaches - see related parts ISO 16000-16, -17, -18 for method details).
- Measurement strategy: Selection of appropriate procedures based on measurement objectives, site conditions and sample types; planning to reduce sampling stress and preserve biological integrity.
- Quality requirements and uncertainty: Emphasis on quality assurance, documentation and consideration of uncertainty to improve comparability between datasets.
- Informative annexes: Includes guidance on moisture-damage indicators, devices for total spore counts and culturable fungi, and a field inspection reporting template to document sampling and potential mould damage.
- Exclusions: Does not cover recording building characteristics in detail, field inspections by qualified professionals, or engineering procedures related to inspections; not intended for occupant exposure quantification.
Applications and who uses it
ISO 16000-19 is designed for professionals involved in indoor air quality (IAQ) and mould assessment:
- Environmental consultants and industrial hygienists conducting mould investigations
- Analytical laboratories performing airborne fungal analyses
- Facility managers and building owners seeking standardized assessment methods
- Public health officers and regulators requiring comparable sampling protocols
- Building remediation contractors planning investigation and verification sampling
Practical uses include identifying active mould growth sources, planning sampling campaigns, standardizing laboratory reporting and comparing results across sites or over time. Note: prior field inspection by qualified personnel is required before microbiological sampling.
Related standards
Keywords: ISO 16000-19:2012, indoor air, sampling strategy for moulds, mould sampling, fungal detection, indoor air quality, mould spores, ISO 16000.
