ISO 17993:2002 PDF

Water quality — Determination of 15 polycyclic aromatic hydrocarbons (PAH) in water by HPLC with fluorescence detection after liquid-liquid extraction

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Title (English):

ISO 17993:2002

Water quality — Determination of 15 polycyclic aromatic hydrocarbons (PAH) in water by HPLC with fluorescence detection after liquid-liquid extraction

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 20
Publication date:: August 29, 2002
Edition:: ISO IS 17993 edition 1 version 1
ICS:: 13.060.50

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Description (English):

ISO 17993:2002 specifies a method using high performance liquid chromatography (HPLC) with fluorescence detection for the determination of 15 selected polycyclic aromatic hydrocarbons (PAH) [naphthalene, acenaphthene, phenanthrene, fluoranthene, benzo(a)anthracene, benzo(b)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, fluorene, anthracene, pyrene, chrysene, benzo(k)fluoranthene, indeno(1,2,3-cd)pyrene, benzo(ghi)perylene] in drinking and ground water in mass concentrations greater than 0,005 microgram/litre (for each single compound) and surface waters in mass concentrations above 0,01 microgram/litre. This method is, with some modification, also suitable for the analysis of waste water. This method may be applicable to other PAH, provided the method is validated for each case.

Technical details

Technical committee: ISO/TC 147/SC 2 - Physical, chemical and biochemical methods
SKU: ISO 17993:2002

Abstract

Overview

ISO 17993:2002 specifies a validated laboratory method for the determination of 15 polycyclic aromatic hydrocarbons (PAH) in water using liquid–liquid extraction followed by high performance liquid chromatography (HPLC) with fluorescence detection. The standard targets drinking and ground water at concentrations above 0.005 µg/L (per compound) and surface waters above 0.01 µg/L (per compound). The method can be adapted for wastewater and for other PAH when appropriately validated.

Determined PAH: naphthalene, acenaphthene, phenanthrene, fluoranthene, benzo(a)anthracene, benzo(b)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, fluorene, anthracene, pyrene, chrysene, benzo(k)fluoranthene, indeno(1,2,3-cd)pyrene, benzo(ghi)perylene.

Key topics and technical requirements

Sample handling & preservation: Use glass or steel containers, avoid plastics/silicones, protect samples from light, dechlorinate (e.g., sodium thiosulfate) and follow ISO 5667 sampling guidance.
Extraction: Liquid–liquid extraction (hexane or equivalent solvent), concentration by evaporation, and optional silica cleanup for contaminated samples.
Chromatography: HPLC separation on suitable columns using gradient elution; fluorescence detection with programmed excitation/emission wavelengths for selective identification and quantification.
Instrumentation & accessories: HPLC with fluorescence detector (wavelength programming), degassing, binary pumps, column thermostat (±0.5 °C), autosampler vials, microfilters (0.45 µm) and silica cartridges for cleanup.
Standards & calibration: Use certified single and multi-compound stock solutions (examples: 10 µg/mL), prepare at least five calibration levels; monitor method blanks and quality control.
Interferences & limitations: Fluorescent co-eluting compounds, solvent residues and unresolved peaks can affect results (notably for naphthalene, phenanthrene, dibenzo(ah)anthracene/indeno-pyrene). Acenaphthylene is excluded because it is non-fluorescent.
Safety: Many PAH are suspected carcinogens; solvents such as acetonitrile, hexane and dichloromethane are toxic-apply proper lab safety.

Practical applications

Routine monitoring of drinking water, groundwater and surface water for regulatory compliance and public health protection.
Environmental laboratories performing PAH screening and confirmation at low ng/L levels.
Water utilities, environmental agencies, remediation contractors, and researchers assessing contamination sources, treatment efficiency, or compliance with limits (e.g., EU Council Directive 98/83/EC for benzo(a)pyrene).

Related standards

ISO 5667-2 and ISO 5667-3 - sampling techniques and preservation for water quality
ISO 8466-1 - calibration and evaluation of analytical methods

Keywords: ISO 17993:2002, PAH determination, HPLC fluorescence, liquid-liquid extraction, water quality, polycyclic aromatic hydrocarbons, drinking water analysis.

Related standards

Standards mentioned in the description

Abstract

Overview

Key topics and technical requirements

Sample handling & preservation: Use glass or steel containers, avoid plastics/silicones, protect samples from light, dechlorinate (e.g., sodium thiosulfate) and follow ISO 5667 sampling guidance.
Extraction: Liquid–liquid extraction (hexane or equivalent solvent), concentration by evaporation, and optional silica cleanup for contaminated samples.
Chromatography: HPLC separation on suitable columns using gradient elution; fluorescence detection with programmed excitation/emission wavelengths for selective identification and quantification.
Instrumentation & accessories: HPLC with fluorescence detector (wavelength programming), degassing, binary pumps, column thermostat (±0.5 °C), autosampler vials, microfilters (0.45 µm) and silica cartridges for cleanup.
Standards & calibration: Use certified single and multi-compound stock solutions (examples: 10 µg/mL), prepare at least five calibration levels; monitor method blanks and quality control.
Interferences & limitations: Fluorescent co-eluting compounds, solvent residues and unresolved peaks can affect results (notably for naphthalene, phenanthrene, dibenzo(ah)anthracene/indeno-pyrene). Acenaphthylene is excluded because it is non-fluorescent.
Safety: Many PAH are suspected carcinogens; solvents such as acetonitrile, hexane and dichloromethane are toxic-apply proper lab safety.

Practical applications

Routine monitoring of drinking water, groundwater and surface water for regulatory compliance and public health protection.
Environmental laboratories performing PAH screening and confirmation at low ng/L levels.
Water utilities, environmental agencies, remediation contractors, and researchers assessing contamination sources, treatment efficiency, or compliance with limits (e.g., EU Council Directive 98/83/EC for benzo(a)pyrene).

Related standards

ISO 5667-2 and ISO 5667-3 - sampling techniques and preservation for water quality
ISO 8466-1 - calibration and evaluation of analytical methods

Keywords: ISO 17993:2002, PAH determination, HPLC fluorescence, liquid-liquid extraction, water quality, polycyclic aromatic hydrocarbons, drinking water analysis.

ISO 17993:2002 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Practical applications

Related standards

Related standards

Abstract

Overview

Key topics and technical requirements

Practical applications

Related standards

Related standards

Water quality - Sampling - Part 3: Preservation and handling of water samples (ISO 5667-3:2024)

Water quality - Sampling - Part 24: Guidance on the auditing of water quality sampling

Water quality -- Calibration and evaluation of analytical methods and estimation of performance characteristi…