ISO 17995:2019 PDF

Water quality — Detection and enumeration of thermotolerant Campylobacter spp

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Title (English):

ISO 17995:2019

Water quality — Detection and enumeration of thermotolerant Campylobacter spp

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 30
Publication date:: November 18, 2019
Edition:: ISO IS 17995 edition 2 version 1
ICS:: 07.100.20

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Description (English):

This document specifies a method for the detection, semi-quantitative and quantitative (MPN) enumeration of thermotolerant Campylobacter species. The method can be applied to all kinds of waters including: drinking water, ground water and well water, fresh, brackish and saline surface water, swimming pools, spa and hydrotherapy pools, recreational waters, agricultural waters and runoff, untreated and treated wastewater and also sand and other sediments. This method can be used for the detection of Campylobacter species in a specified sample volume. Clean water samples with low turbidity can be membrane filtered for either a qualitative method, semi-quantitative or quantitative (MPN) method. Water samples with higher turbidity, such as primary and secondary wastewater effluents and sediments, are analysed using the same qualitative, semi-quantitative or quantitative MPN method by direct inoculation of material into bottles or tubes. Sediments can be suspended in a suitable diluent or inoculated directly into enrichment broths. Users wishing to employ this method are expected to verify its performance for the particular matrix under their own laboratory conditions.

Technical details

Technical committee: ISO/TC 147/SC 4 - Microbiological methods
SKU: ISO 17995:2019

Abstract

Overview

ISO 17995:2019 - Water quality: Detection and enumeration of thermotolerant Campylobacter spp. specifies a standardized microbiological method for detecting, semi‑quantitatively and quantitatively (MPN) enumerating thermotolerant Campylobacter species in a wide range of water and sediment matrices. The method covers sample processing (membrane filtration or direct inoculation), selective enrichment, isolation on modified charcoal cefoperazone deoxycholate agar (mCCDA), and confirmation/optional speciation steps.

Key technical topics and requirements

Scope: Applicable to drinking water, groundwater, surface waters (fresh, brackish, saline), recreational waters (pools, spas), agricultural runoff, treated/untreated wastewater, sand and sediments.
Sample processing:
- Low‑turbidity (clean) waters: membrane filtration for qualitative, semi‑quantitative or MPN methods.
- High‑turbidity waters and sediments: direct inoculation into enrichment broths or suspension of sediments in diluent prior to enrichment.
Enrichment media:
- Bolton broth for cleaner samples.
- Preston broth for heavily contaminated samples.
- Incubation microaerobically at (37 ± 1) °C for (44 ± 4) h; sample:broth ratio ≤ 10% for single‑strength broths.
Isolation and enumeration:
- Isolation on mCCDA incubated at (41.5 ± 1) °C for (44 ± 4) h in a microaerobic atmosphere.
- Semi‑quantitative uses at least three 10‑fold volumes (e.g., 10 ml, 100 ml, 1000 ml).
- Quantitative enumeration uses a Most Probable Number (MPN) approach (e.g., 500 ml, 5×100 ml, 5×10 ml).
Confirmation and optional speciation:
- Presumptive colonies confirmed by morphology, motility, inability to grow aerobically at 25 °C and biochemical tests.
- Optional species identification tests include catalase, hippurate and indoxyl acetate reactions.
Quality assurance: Users must verify method performance for their specific matrix and laboratory conditions.

Practical applications - who uses ISO 17995:2019

Public health and environmental microbiology laboratories monitoring waterborne pathogens.
Drinking water regulators and utilities assessing source water and distribution safety.
Wastewater treatment operators and agricultural monitoring programs tracking contamination and risk.
Recreational water managers (pools, spas, beaches) investigating outbreaks or compliance.
Research institutions studying Campylobacter ecology, survival, and transmission in aquatic environments.

Related standards

ISO 8199 - General requirements and guidance for microbiological examinations by culture.
ISO 11133 - Preparation, production, storage and performance testing of culture media.
ISO 19458 - Water quality - Sampling for microbiological analysis.

ISO 17995:2019 provides a harmonized, defensible approach for detecting and enumerating thermotolerant Campylobacter spp. in diverse water matrices, supporting surveillance, outbreak investigation and regulatory compliance.

Related standards

Standards mentioned in the description

Abstract

Overview

Key technical topics and requirements

Scope: Applicable to drinking water, groundwater, surface waters (fresh, brackish, saline), recreational waters (pools, spas), agricultural runoff, treated/untreated wastewater, sand and sediments.
Sample processing:
- Low‑turbidity (clean) waters: membrane filtration for qualitative, semi‑quantitative or MPN methods.
- High‑turbidity waters and sediments: direct inoculation into enrichment broths or suspension of sediments in diluent prior to enrichment.
Enrichment media:
- Bolton broth for cleaner samples.
- Preston broth for heavily contaminated samples.
- Incubation microaerobically at (37 ± 1) °C for (44 ± 4) h; sample:broth ratio ≤ 10% for single‑strength broths.
Isolation and enumeration:
- Isolation on mCCDA incubated at (41.5 ± 1) °C for (44 ± 4) h in a microaerobic atmosphere.
- Semi‑quantitative uses at least three 10‑fold volumes (e.g., 10 ml, 100 ml, 1000 ml).
- Quantitative enumeration uses a Most Probable Number (MPN) approach (e.g., 500 ml, 5×100 ml, 5×10 ml).
Confirmation and optional speciation:
- Presumptive colonies confirmed by morphology, motility, inability to grow aerobically at 25 °C and biochemical tests.
- Optional species identification tests include catalase, hippurate and indoxyl acetate reactions.
Quality assurance: Users must verify method performance for their specific matrix and laboratory conditions.

Practical applications - who uses ISO 17995:2019

Public health and environmental microbiology laboratories monitoring waterborne pathogens.
Drinking water regulators and utilities assessing source water and distribution safety.
Wastewater treatment operators and agricultural monitoring programs tracking contamination and risk.
Recreational water managers (pools, spas, beaches) investigating outbreaks or compliance.
Research institutions studying Campylobacter ecology, survival, and transmission in aquatic environments.

Related standards

ISO 8199 - General requirements and guidance for microbiological examinations by culture.
ISO 11133 - Preparation, production, storage and performance testing of culture media.
ISO 19458 - Water quality - Sampling for microbiological analysis.

ISO 17995:2019 PDF

Technical details

Abstract

Overview

Key technical topics and requirements

Practical applications - who uses ISO 17995:2019

Related standards

Related standards

Abstract

Overview

Key technical topics and requirements

Practical applications - who uses ISO 17995:2019

Related standards

Related standards

Water quality - General requirements and guidance for microbiological examinations by culture (ISO 8199:2018)

Microbiology of food, animal feed and water - Preparation, production, storage and performance testing of cul…

Water quality - Sampling for microbiological analysis (ISO 19458:2006)