ISO 23611-4:2022 PDF

Soil quality — Sampling of soil invertebrates — Part 4: Sampling, extraction and identification of soil-inhabiting nematodes

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Title (English):

ISO 23611-4:2022

Soil quality — Sampling of soil invertebrates — Part 4: Sampling, extraction and identification of soil-inhabiting nematodes

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 27
Publication date:: August 1, 2022
Edition:: ISO IS 23611 edition 2 version 1
ICS:: 13.080.05

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Description (English):

This document specifies a method for sampling and handling free-living nematodes from terrestrial field soils as a prerequisite for using them as bio-indicators (e.g. to assess the quality of a soil as a habitat for organisms). This document applies to all terrestrial biotopes in which nematodes occur. The sampling design of field studies in general is specified in ISO 18400-101. This document is not applicable to aquatic nematodes because of differences in the sample matrix (e.g. water column). Methods for some other soil organism groups such as earthworms, collembolans enchytraeids or macro-invertebrates are covered in ISO 23611-1, ISO 23611-2, ISO 23611-3 and ISO 23611-5. This document does not cover the pedological characterization of the site which is highly recommendable when sampling soil invertebrates. ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461 and ISO 11465 include suitable procedures for measuring pH, particle size distribution, C/N ratio, organic carbon content and water-holding capacity.

Technical details

Technical committee: ISO/TC 190/SC 4 - Biological characterization
SKU: ISO 23611-4:2022

Abstract

Overview

ISO 23611-4:2022 - Soil quality - Sampling of soil invertebrates - Part 4 - specifies a standardized method for sampling, extraction and identification of soil‑inhabiting nematodes from terrestrial field soils. The standard defines field sampling, laboratory extraction (recommended: Oostenbrink elutriator method), counting, fixation and identification procedures as a prerequisite for using nematodes as bio‑indicators of soil quality. It applies to all terrestrial biotopes where nematodes occur and excludes aquatic nematodes.

Key topics and technical requirements

Sampling design
- Use small cylindrical cores (≈2 cm diameter; 10 or 15 cm length) or auger; combine cores into a bulk‑sample for homogeneous areas.
- Protect samples from temperature fluctuations, water loss and mechanical disturbance during transport and storage (storage recommended ≤4 weeks at 4 °C).
Extraction principle
- Recommended Oostenbrink elutriator (model III) for routine extractions: uses upward water current to suspend nematodes, followed by sieving (three sieves, mesh ~45 µm) and transfer to a cotton‑wool filter for active movement separation.
- Suitable soil subsample sizes for Oostenbrink: typically 100–500 g.
- Alternative methods (e.g., Baermann funnel/tray) are discussed in informative annexes.
Post‑extraction processing
- Produce a 100 ml nematode suspension; count in two 10 ml aliquots.
- Concentrate, fix and mount nematodes on mass slides (300–400 individuals per slide).
- Identify a minimum number of individuals (e.g., at least 150 or a defined percentage of the sample) to family/genus/species using morphological keys.
Ecological assessment
- Uses ecological classifications such as the colonizer–persister (cp) scale and indices like the Maturity Index for soil quality assessment.

Applications

Environmental monitoring and long‑term soil biodiversity surveys
Soil quality and habitat assessment using nematode community structure
Regulatory and impact studies (e.g., evaluation of chemical effects on soil fauna)
Agricultural advisory and phytosanitary investigations
Research in nematology, soil ecology and bio‑indication

Who should use it

Soil scientists, ecologists and nematologists
Environmental monitoring laboratories and biodiversity assessment programs
Agricultural consultants and phytosanitary services
Regulatory agencies and remediation project teams

Related standards

ISO 18400‑101 (sampling design for field studies)
Other ISO 23611 parts (earthworms, collembolans, enchytraeids, macro‑invertebrates)
Soil property standards: ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461, ISO 11465
Relevant testing: ISO 11268‑3

Keywords: ISO 23611-4:2022, soil quality, nematode sampling, nematode extraction, Oostenbrink elutriator, soil bio-indicators, mass slide, Baermann funnel.

Related standards

Standards mentioned in the description

Abstract

Overview

Key topics and technical requirements

Sampling design
- Use small cylindrical cores (≈2 cm diameter; 10 or 15 cm length) or auger; combine cores into a bulk‑sample for homogeneous areas.
- Protect samples from temperature fluctuations, water loss and mechanical disturbance during transport and storage (storage recommended ≤4 weeks at 4 °C).
Extraction principle
- Recommended Oostenbrink elutriator (model III) for routine extractions: uses upward water current to suspend nematodes, followed by sieving (three sieves, mesh ~45 µm) and transfer to a cotton‑wool filter for active movement separation.
- Suitable soil subsample sizes for Oostenbrink: typically 100–500 g.
- Alternative methods (e.g., Baermann funnel/tray) are discussed in informative annexes.
Post‑extraction processing
- Produce a 100 ml nematode suspension; count in two 10 ml aliquots.
- Concentrate, fix and mount nematodes on mass slides (300–400 individuals per slide).
- Identify a minimum number of individuals (e.g., at least 150 or a defined percentage of the sample) to family/genus/species using morphological keys.
Ecological assessment
- Uses ecological classifications such as the colonizer–persister (cp) scale and indices like the Maturity Index for soil quality assessment.

Applications

Environmental monitoring and long‑term soil biodiversity surveys
Soil quality and habitat assessment using nematode community structure
Regulatory and impact studies (e.g., evaluation of chemical effects on soil fauna)
Agricultural advisory and phytosanitary investigations
Research in nematology, soil ecology and bio‑indication

Who should use it

Soil scientists, ecologists and nematologists
Environmental monitoring laboratories and biodiversity assessment programs
Agricultural consultants and phytosanitary services
Regulatory agencies and remediation project teams

Related standards

ISO 18400‑101 (sampling design for field studies)
Other ISO 23611 parts (earthworms, collembolans, enchytraeids, macro‑invertebrates)
Soil property standards: ISO 10390, ISO 10694, ISO 11272, ISO 11274, ISO 11277, ISO 11461, ISO 11465
Relevant testing: ISO 11268‑3

Keywords: ISO 23611-4:2022, soil quality, nematode sampling, nematode extraction, Oostenbrink elutriator, soil bio-indicators, mass slide, Baermann funnel.

ISO 23611-4:2022 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Applications

Who should use it

Related standards

Related standards

Abstract

Overview

Key topics and technical requirements

Applications

Who should use it

Related standards

Related standards

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