Overview
EN ISO 16187:2025 (ISO 16187:2025) specifies quantitative laboratory test methods to assess the antibacterial activity of footwear and footwear components. Adopted by CEN as EN ISO 16187:2025, the standard applies to all types of footwear and components that use non‑diffusing antibacterial treatments. It replaces EN ISO 16187:2013 / ISO 16187:2013 and defines procedures for measuring and reporting antibacterial performance in a reproducible way.
Key topics and requirements
- Scope and safety: The document includes a clear safety caution - tests use live bacteria and must be carried out in appropriate containment facilities by trained personnel.
- Test principles: Quantitative evaluation of antibacterial activity using controlled inocula and contact between microorganisms and the treated material.
- Test methods (normative annexes):
- Static challenge test (Annex A)
- Film contact method (Annex B)
- Dynamic challenge test (Annex C)
- Test setup components:
- Apparatus and environmental conditions
- Reagents and culture media (e.g., nutrient broth/agar; alternatives such as tryptic soy media are noted)
- Test microorganisms (specified strains and storage)
- Preparation of inocula and test specimens, including pre‑treatment
- Test procedure and result expression:
- Enumeration of viable bacteria and calculation methods
- Antibacterial activity ratio and criteria for judging test effectiveness
- Required content for the test report
- Terminology and updates: The 2025 edition adds terms (e.g., “neutralizer”), updates media options (TSA/TSB alternatives), and revises normative references.
Practical applications and users
Who uses ISO 16187:2025:
- Footwear manufacturers developing or validating antibacterial treatments (insoles, linings, uppers)
- Independent testing laboratories performing product certification, quality control, or comparative testing
- R&D teams evaluating new antimicrobial treatments and formulations
- Procurement and regulatory bodies requiring documented antibacterial performance for specifications or claims
- Standards bodies and conformity assessors
Typical uses:
- Verifying antibacterial performance claims
- Routine quality control during production
- Comparing treatment options in product development
- Supporting compliance for market access and technical specifications
Related standards
Keywords: ISO 16187, EN ISO 16187:2025, antibacterial footwear test, footwear antibacterial activity, static challenge test, film contact method, dynamic challenge test, non‑diffusing antibacterial treatments.
