Overview
EN ISO 21149:2017/A1:2022 is Amendment 1 to ISO 21149:2017, adopted by CEN as EN ISO 21149:2017/A1:2022. The standard addresses cosmetics microbiology - enumeration and detection of aerobic mesophilic bacteria. The amendment updates media and preparation details for enrichment and agar media used in the recovery and enumeration of aerobic mesophilic bacteria from cosmetic products and related samples.
Key topics and requirements
- Scope: Procedures for detection and enumeration of aerobic mesophilic bacteria in cosmetics (as part of ISO 21149 method family).
- Enrichment broth: Amendment clarifies subclause titles and details for enrichment media.
- Eugon LT100 broth: Described with neutralizers (lecithin, polysorbate 80) and a dispersing agent (octoxynol 9).
- Modified Eugon LT broth (new): Specification of composition per 1 000 ml:
- Pancreatic digest of casein 15 g
- Papain (papaic) digest of soybean meal 5 g
- Sodium chloride 4 g
- L-cystine 0.7 g
- Sodium sulphite 0.2 g
- Glucose 5.5 g
- Egg lecithin 1 g
- Polysorbate 80 15 g
- Sodium lauryl ether sulfate 1.56 g
- Water to 1 000 ml
- Preparation: Dissolve polysorbate 80 and egg lecithin successively into boiling water; dissolve other components while heating; sterilize at 121 °C for 15 min; mix while hot; final pH 7.0 ± 0.2 at room temperature.
- Agar media: New Modified Eugon LT agar composition mirrors the broth with added agar (15 g per 1 000 ml) and identical preparation/sterilization instructions.
- Document control: Amendment was approved 10 September 2022; CEN endorsed it without modification and member bodies were to implement by March 2023.
Applications
- Routine microbial quality control (QC) of cosmetic raw materials, in-process samples and finished products.
- Validation and verification of preservative efficacy and microbial limit testing.
- Development and standardization of laboratory methods for cosmetic microbiology (culture-based enumeration and detection).
Who should use this standard
- Cosmetic industry microbiologists and QC laboratories
- Contract testing laboratories performing cosmetic microbial analyses
- Regulatory authorities and auditors assessing compliance with microbial safety requirements
- Standards bodies and method developers in cosmetics microbiology
Related standards
Keywords: ISO 21149, EN ISO 21149, cosmetics microbiology, aerobic mesophilic bacteria, Eugon LT broth, modified Eugon LT agar, microbial enumeration, cosmetic QC, culture media.
