ISO 13906:2008 PDF

Animal feeding stuffs — Determination of acid detergent fibre (ADF) and acid detergent lignin (ADL) contents

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Title (English):

ISO 13906:2008

Animal feeding stuffs — Determination of acid detergent fibre (ADF) and acid detergent lignin (ADL) contents

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 17
Publication date:: July 2, 2008
Edition:: ISO IS 13906 edition 1 version 1
ICS:: 65.120

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Description (English):

ISO 13906:2008 specifies a method for the determination of acid detergent fibre (ADF) insoluble residue and acid detergent lignin (ADL) in all types of animal feeding stuffs. The limit of determination is 1 % mass fraction for ADF and 1,5 % mass fraction for ADL. A gravimetric routine and reference method is used.

Technical details

Technical committee: ISO/TC 34/SC 10 - Animal feeding stuffs
SKU: ISO 13906:2008

Abstract

Overview

ISO 13906:2008 specifies a gravimetric routine and reference method for determining acid detergent fibre (ADF) and acid detergent lignin (ADL) in all types of animal feeding stuffs. The standard defines the chemical principle, required reagents and apparatus, sample preparation and testing procedure. Limits of determination are 1 % mass fraction for ADF and 1.5 % mass fraction for ADL. The method is widely used in feed analysis laboratories for reliable measurement of fibrous components that affect digestibility and feed quality.

Key Topics

Analytical principle: ADF is the insoluble residue after treatment with a cationic detergent in 0.5 mol/l sulfuric acid (primarily cellulose, lignin and insoluble protein complexes). ADL is the residue remaining after treatment with 72 % (12.00 mol/l) sulfuric acid, which solubilizes cellulose and leaves lignin.
Reagents: Acid detergent solution (cetyl(trimethyl)ammonium bromide in 0.5 mol/l H2SO4), 72 % H2SO4, acetone, diatomaceous earth (filtration aid), n‑octanol (antifoam).
Apparatus: Analytical balances, mills (target particle size 0.22–0.26 mm), air‑ventilated and vacuum ovens, refluxing or Fibertec‑type extraction units, fritted‑disk crucibles, vacuum filtration manifold, incineration furnace.
Sample preparation: Follow ISO 6498 for test sample preparation; dry samples to

Related standards

Mentioned in the description and other ISO standards

Overview

Key Topics

Analytical principle: ADF is the insoluble residue after treatment with a cationic detergent in 0.5 mol/l sulfuric acid (primarily cellulose, lignin and insoluble protein complexes). ADL is the residue remaining after treatment with 72 % (12.00 mol/l) sulfuric acid, which solubilizes cellulose and leaves lignin.
Reagents: Acid detergent solution (cetyl(trimethyl)ammonium bromide in 0.5 mol/l H2SO4), 72 % H2SO4, acetone, diatomaceous earth (filtration aid), n‑octanol (antifoam).
Apparatus: Analytical balances, mills (target particle size 0.22–0.26 mm), air‑ventilated and vacuum ovens, refluxing or Fibertec‑type extraction units, fritted‑disk crucibles, vacuum filtration manifold, incineration furnace.
Sample preparation: Follow ISO 6498 for test sample preparation; dry samples to

ISO 13906:2008 PDF

Technical details

Abstract

Overview

Key Topics

Related standards

Abstract

Overview

Key Topics

Related standards

Animal feeding stuffs - Guidelines for sample preparation (ISO 6498:2012)

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