ISO 15685:2012 PDF

Soil quality — Determination of potential nitrification and inhibition of nitrification — Rapid test by ammonium oxidation

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Title (English):

ISO 15685:2012

Soil quality — Determination of potential nitrification and inhibition of nitrification — Rapid test by ammonium oxidation

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 11
Publication date:: July 10, 2012
Edition:: ISO IS 15685 edition 2 version 1
ICS:: 13.080.30

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Description (English):

ISO 15685:2012 specifies a rapid method for the determination of the potential rate of ammonium oxidation and inhibition of nitrification in soils. This method is suitable for all soils containing a population of nitrifying microorganisms. It can be used as a rapid screening test for monitoring soil quality and quality of wastes, and is suitable for testing the effects of cultivation methods, chemical substances [except volatiles i.e. H > 1 (Henry's constant)], extracts of biosolids and pollution in soils.

Technical details

Technical committee: ISO/TC 190/SC 4 - Biological characterization
SKU: ISO 15685:2012

Abstract

Overview

ISO 15685:2012 - "Soil quality - Determination of potential nitrification and inhibition of nitrification - Rapid test by ammonium oxidation" specifies a rapid laboratory method to measure the potential rate of ammonium oxidation and the inhibition of nitrification in soils. The test is applicable to any soil containing nitrifying microorganisms and is designed as a screening tool for soil quality monitoring, waste characterization and assessing effects of cultivation, chemicals (non‑volatile), biosolid extracts and pollution on nitrification.

Key topics and technical requirements

Principle: Ammonium-oxidizing bacteria in a buffered soil slurry (pH ~7.2) are supplied with ammonium (diammonium sulfate). Nitrite-oxidizing bacteria are inhibited with sodium chlorate so that accumulated nitrite reflects ammonium oxidation over a typical 6 h incubation.
Measurement: Nitrite concentration is measured (e.g., automated segmented flow analysis) and converted to a rate (ng NO2‑N/g dry soil/h). Inhibition is expressed as percentage change vs control; inhibitory dose values (e.g., ID25, ID50) can be derived.
Test conditions: Typical incubation at (25 ± 2) °C with orbital shaking; sampling commonly at 2 h and 6 h if oxidation is linear. Test medium contains a phosphate buffer, sodium chlorate (5–15 mmol/L) and ammonium source.
Design: Replication (minimum three replicates per treatment), controls, and range‑finding for chemical concentrations (at least five concentrations in geometric progression) are required. Vehicles for poorly soluble chemicals must be controlled.
Sample handling: Guidance on sampling, storage and characterization is referenced (ISO 10381‑6). Recommended soil descriptors include pH, organic matter, total N, particle size, water content and cation exchange capacity.
Limitations: Volatile test substances are excluded from this standard; substances active at pH

Related standards

Mentioned in the description and other ISO standards

Overview

Key topics and technical requirements

Principle: Ammonium-oxidizing bacteria in a buffered soil slurry (pH ~7.2) are supplied with ammonium (diammonium sulfate). Nitrite-oxidizing bacteria are inhibited with sodium chlorate so that accumulated nitrite reflects ammonium oxidation over a typical 6 h incubation.
Measurement: Nitrite concentration is measured (e.g., automated segmented flow analysis) and converted to a rate (ng NO2‑N/g dry soil/h). Inhibition is expressed as percentage change vs control; inhibitory dose values (e.g., ID25, ID50) can be derived.
Test conditions: Typical incubation at (25 ± 2) °C with orbital shaking; sampling commonly at 2 h and 6 h if oxidation is linear. Test medium contains a phosphate buffer, sodium chlorate (5–15 mmol/L) and ammonium source.
Design: Replication (minimum three replicates per treatment), controls, and range‑finding for chemical concentrations (at least five concentrations in geometric progression) are required. Vehicles for poorly soluble chemicals must be controlled.
Sample handling: Guidance on sampling, storage and characterization is referenced (ISO 10381‑6). Recommended soil descriptors include pH, organic matter, total N, particle size, water content and cation exchange capacity.
Limitations: Volatile test substances are excluded from this standard; substances active at pH

ISO 15685:2012 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Related standards

Abstract

Overview

Key topics and technical requirements

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