ISO 16665:2014 PDF

Water quality — Guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna

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Title (English):

ISO 16665:2014

Water quality — Guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 33
Publication date:: January 6, 2014
Edition:: ISO IS 16665 edition 2 version 1
ICS:: 13.060.10

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Description (English):

ISO 16665:2014 provides guidelines on the quantitative collection and processing of subtidal soft-bottom macrofaunal samples in marine waters. ISO 16665:2014 encompasses: a) development of the sampling programme; b) requirements for sampling equipment; c) sampling and sample treatment in the field; d) sorting and species identification; e) storage of collected and processed material. ISO 16665:2014 does not specifically address the following, although some elements may be applicable: bioassay sub-sampling; deep water (>750 m) or offshore sampling; in situ faunal studies, e.g. recolonization assays; non-benthic organisms caught in the sampling device; estuarine sampling; intertidal sampling; meiofaunal sampling and analysis; sampling by dredge and sledge; self-contained underwater breathing apparatus (SCUBA) sampling; statistical design. Accuracy of position fixing is determined by the geographical area, equipment used and survey objective.

Technical details

Technical committee: ISO/TC 147/SC 5 - Biological methods
SKU: ISO 16665:2014

Abstract

Overview

ISO 16665:2014 - Water quality: Guidelines for quantitative sampling and sample processing of marine soft-bottom macrofauna - provides standardized guidance for collecting and processing subtidal soft‑bottom macrofaunal samples in marine waters. The standard covers development of a sampling programme, equipment requirements, field sampling and treatment, sorting and species identification, and storage/archiving of collected material. It is intended to support reliable, comparable data for marine environmental assessment and monitoring (including use with the EU Water Framework Directive).

Key topics and requirements

Sampling programme and station positioning: guidance on designing a sampling plan, selecting sampling stations and reference stations, and documenting station coordinates and depth. (Note: accuracy of position fixing depends on area, equipment and objectives.)
Sampling equipment: minimum requirements for grab and coring devices used to collect benthic macrofauna retained on 0.5 mm or 1 mm mesh screens.
Field sampling and sample treatment: procedures for sample collection, immediate handling, fixation and recording of environmental descriptors (e.g., sediment and water properties).
Sample processing and laboratory methods: sorting protocols, sample residue handling, taxon determination, quantification (counts, biomass) and reference collections.
Quality assurance / quality control (QA/QC): training, equipment calibration, auditing, checklists, identification literature and procedures to ensure intercomparability between operators and surveys.
Data reporting and storage: recommendations for reporting taxonomic lists, abundance, biomass and for archiving specimens and metadata.
Scope limits and exclusions: does not specifically address deep water (>750 m), estuarine or intertidal sampling, meiofauna, dredge/ sledge sampling, SCUBA sampling, bioassay sub-sampling or statistical design.

Practical applications

Standardizes quantitative sampling methods for benthic macrofauna used in environmental impact assessments, routine monitoring, habitat mapping and pollution studies.
Supports comparability of datasets over time and between organizations, improving reliability of biological indicators for water quality and sediment condition assessments.
Informs operational procedures for field teams, laboratory taxonomists and data managers responsible for benthic community analysis.

Who uses ISO 16665:2014

Marine ecologists and benthic taxonomists
Environmental monitoring agencies and contractors
Consultants performing marine impact assessments
Research institutions conducting surveys of soft‑bottom macrofauna
Regulatory bodies implementing monitoring programs (e.g., related to the EU Water Framework Directive)

Related standards

ISO 5667 series (water quality sampling standards) - referenced for related sampling terminology and methods.

Related standards

Mentioned in the description and other ISO standards

Abstract

Overview

Key topics and requirements

Sampling programme and station positioning: guidance on designing a sampling plan, selecting sampling stations and reference stations, and documenting station coordinates and depth. (Note: accuracy of position fixing depends on area, equipment and objectives.)
Sampling equipment: minimum requirements for grab and coring devices used to collect benthic macrofauna retained on 0.5 mm or 1 mm mesh screens.
Field sampling and sample treatment: procedures for sample collection, immediate handling, fixation and recording of environmental descriptors (e.g., sediment and water properties).
Sample processing and laboratory methods: sorting protocols, sample residue handling, taxon determination, quantification (counts, biomass) and reference collections.
Quality assurance / quality control (QA/QC): training, equipment calibration, auditing, checklists, identification literature and procedures to ensure intercomparability between operators and surveys.
Data reporting and storage: recommendations for reporting taxonomic lists, abundance, biomass and for archiving specimens and metadata.
Scope limits and exclusions: does not specifically address deep water (>750 m), estuarine or intertidal sampling, meiofauna, dredge/ sledge sampling, SCUBA sampling, bioassay sub-sampling or statistical design.

Practical applications

Standardizes quantitative sampling methods for benthic macrofauna used in environmental impact assessments, routine monitoring, habitat mapping and pollution studies.
Supports comparability of datasets over time and between organizations, improving reliability of biological indicators for water quality and sediment condition assessments.
Informs operational procedures for field teams, laboratory taxonomists and data managers responsible for benthic community analysis.

Who uses ISO 16665:2014

Marine ecologists and benthic taxonomists
Environmental monitoring agencies and contractors
Consultants performing marine impact assessments
Research institutions conducting surveys of soft‑bottom macrofauna
Regulatory bodies implementing monitoring programs (e.g., related to the EU Water Framework Directive)

Related standards

ISO 5667 series (water quality sampling standards) - referenced for related sampling terminology and methods.

ISO 16665:2014 PDF

Technical details

Abstract

Overview

Key topics and requirements

Practical applications

Who uses ISO 16665:2014

Related standards

Related standards

Abstract

Overview

Key topics and requirements

Practical applications

Who uses ISO 16665:2014

Related standards

Related standards

Water quality - Sampling - Part 3: Preservation and handling of water samples (ISO 5667-3:2024)

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