ISO 18609:2000 PDF

Animal and vegetable fats and oils — Determination of unsaponifiable matter — Method using hexane extraction

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Title (English):

ISO 18609:2000

Animal and vegetable fats and oils — Determination of unsaponifiable matter — Method using hexane extraction

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 8
Publication date:: August 24, 2000
Edition:: ISO IS 18609 edition 1 version 1
ICS:: 67.200.10

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Description (English):

La présente Norme internationale spécifie une méthode à trois extractions à l'hexane pour la détermination de la teneur en matières insaponifiables des corps gras d'origines animale et végétale. Cette méthode est applicable à tous les corps gras mais pas aux cires. ATTENTION - Par rapport à la méthode donnée dans l'ISO 3596, la présente méthode donne des résultats systématiquement faibles.

Technical details

Technical committee: ISO/TC 34/SC 11 - Animal and vegetable fats and oils
SKU: ISO 18609:2000

Abstract

Overview

ISO 18609:2000 specifies a laboratory method for determining the unsaponifiable matter content of animal and vegetable fats and oils using a three-stage hexane extraction. The method covers saponification with ethanolic potassium hydroxide, extraction of non-saponifiable components by hexane (or light petroleum), evaporation of solvent and gravimetric determination of the residue after drying. Note: the method is applicable to fats and oils but not to waxes, and it gives results that are systematically lower than the diethyl-ether method in ISO 3596.

Keywords: ISO 18609:2000, unsaponifiable matter, hexane extraction, animal and vegetable fats and oils, fat analysis, quality control

Key topics and technical requirements

Scope: Determination of unsaponifiable matter in all fats and oils except waxes.
Principle: Boil sample with ethanolic KOH to saponify; extract unsaponifiables with hexane; evaporate and weigh residue.
Reagents: Analytical-grade n-hexane (or light petroleum 40–60 °C), ethanolic KOH (≈1 mol/L), ethanol wash solution, phenolphthalein indicator.
Apparatus: 250 ml round-bottom flask with reflux condenser, 250 ml PTFE-stopcock separating funnels, boiling water bath, oven at 103 ± 2 °C (or vacuum drying).
Procedure highlights:
- Use ~5 g test portion; saponify 1 h under reflux.
- Perform three successive 50 ml hexane extractions; combine extracts.
- Wash extracts with ethanolic solution until neutral (phenolphthalein).
- Evaporate solvent, dry residue at 103 °C and weigh to constant mass (repeat drying until mass change

Related standards

Mentioned in the description and other ISO standards

Overview

Keywords: ISO 18609:2000, unsaponifiable matter, hexane extraction, animal and vegetable fats and oils, fat analysis, quality control

Key topics and technical requirements

Scope: Determination of unsaponifiable matter in all fats and oils except waxes.
Principle: Boil sample with ethanolic KOH to saponify; extract unsaponifiables with hexane; evaporate and weigh residue.
Reagents: Analytical-grade n-hexane (or light petroleum 40–60 °C), ethanolic KOH (≈1 mol/L), ethanol wash solution, phenolphthalein indicator.
Apparatus: 250 ml round-bottom flask with reflux condenser, 250 ml PTFE-stopcock separating funnels, boiling water bath, oven at 103 ± 2 °C (or vacuum drying).
Procedure highlights:
- Use ~5 g test portion; saponify 1 h under reflux.
- Perform three successive 50 ml hexane extractions; combine extracts.
- Wash extracts with ethanolic solution until neutral (phenolphthalein).
- Evaporate solvent, dry residue at 103 °C and weigh to constant mass (repeat drying until mass change

ISO 18609:2000 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Related standards

Abstract

Overview

Key topics and technical requirements

Related standards

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