Overview

ISO 18862:2025 - "Coffee and coffee products - Determination of acrylamide" - specifies validated laboratory methods to measure acrylamide in coffee and coffee-derived products. The standard covers sample extraction with water, clean‑up by solid‑phase extraction (SPE) (and optional Carrez precipitation), and quantification by HPLC‑MS/MS or by GC‑MS after derivatization (bromination). Methods were validated across matrices including roasted coffee, soluble coffee, coffee substitutes and coffee products with a validated concentration range from 53 μg/kg to 612.1 μg/kg.

Key technical topics and requirements

Extraction & clean‑up
- Water extraction or dissolution for soluble products.
- Matrix clean‑up using SPE; Carrez precipitation is included as an option to remove interferents.
Analytical methods
- HPLC‑MS/MS: direct measurement using tandem mass spectrometry.
- GC‑MS after derivatization: requires bromination of acrylamide prior to GC‑MS analysis.
Standards and calibration
- Use of isotopically labelled internal standard (D3‑acrylamide; C‑13 labelled standard is allowed as an alternative).
- Guidance on calibration, linearity, working range, laboratory recovery and measurement uncertainty.
Reagents and safety
- Specifies reagent grades (MS‑grade solvents, water per ISO 3696) and warns about acrylamide hazards - use fume hoods and protective measures.
Validation and performance
- Precision data (repeatability, reproducibility), recovery, and uncertainty guidance are provided.
- Informative annexes include performance characteristics (Annex A), absorber materials (Annex B), example columns/conditions (Annex C) and HPLC‑MS/MS sample prep examples (Annex D).
Documentation
- Requirements for test reports and identification criteria for peak confirmation.

Practical applications and users

Who uses ISO 18862:2025:

Food testing laboratories performing routine acrylamide monitoring in coffee.
Coffee manufacturers and quality‑control teams validating product safety and process changes.
Regulatory agencies and compliance bodies setting or enforcing acrylamide limits.
Research labs studying acrylamide formation, mitigation strategies or processing effects.

Typical uses:

Regulatory compliance testing and reporting.
Internal quality control and supplier verification.
Method transfer, proficiency testing and inter‑laboratory comparisons.
Research into roasting/processing impacts on acrylamide levels.

Related standards

ISO 3696 - Water for analytical laboratory use (referenced for reagent water quality).
The standard is the second edition and replaces ISO 18862:2016; consult national bodies for implementation and patent notices.

Keywords: ISO 18862:2025, acrylamide in coffee, HPLC‑MS/MS, GC‑MS derivatization, solid‑phase extraction (SPE), coffee product testing, acrylamide validation.

Overview

Key technical topics and requirements

Extraction & clean‑up
- Water extraction or dissolution for soluble products.
- Matrix clean‑up using SPE; Carrez precipitation is included as an option to remove interferents.
Analytical methods
- HPLC‑MS/MS: direct measurement using tandem mass spectrometry.
- GC‑MS after derivatization: requires bromination of acrylamide prior to GC‑MS analysis.
Standards and calibration
- Use of isotopically labelled internal standard (D3‑acrylamide; C‑13 labelled standard is allowed as an alternative).
- Guidance on calibration, linearity, working range, laboratory recovery and measurement uncertainty.
Reagents and safety
- Specifies reagent grades (MS‑grade solvents, water per ISO 3696) and warns about acrylamide hazards - use fume hoods and protective measures.
Validation and performance
- Precision data (repeatability, reproducibility), recovery, and uncertainty guidance are provided.
- Informative annexes include performance characteristics (Annex A), absorber materials (Annex B), example columns/conditions (Annex C) and HPLC‑MS/MS sample prep examples (Annex D).
Documentation
- Requirements for test reports and identification criteria for peak confirmation.

Practical applications and users

Who uses ISO 18862:2025:

Food testing laboratories performing routine acrylamide monitoring in coffee.
Coffee manufacturers and quality‑control teams validating product safety and process changes.
Regulatory agencies and compliance bodies setting or enforcing acrylamide limits.
Research labs studying acrylamide formation, mitigation strategies or processing effects.

Typical uses:

Regulatory compliance testing and reporting.
Internal quality control and supplier verification.
Method transfer, proficiency testing and inter‑laboratory comparisons.
Research into roasting/processing impacts on acrylamide levels.

Related standards

ISO 3696 - Water for analytical laboratory use (referenced for reagent water quality).
The standard is the second edition and replaces ISO 18862:2016; consult national bodies for implementation and patent notices.

Keywords: ISO 18862:2025, acrylamide in coffee, HPLC‑MS/MS, GC‑MS derivatization, solid‑phase extraction (SPE), coffee product testing, acrylamide validation.

ISO 18862:2025 PDF

Technical details

Abstract

Overview

Key technical topics and requirements

Practical applications and users

Related standards

Related standards

Abstract

Overview

Key technical topics and requirements

Practical applications and users

Related standards

Related standards

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