ISO 23822:2026 PDF

Eggs and egg products — Determination of nitroimidazole residues — Liquid chromatography-tandem mass spectrometry method

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Title (English):

ISO 23822:2026

Eggs and egg products — Determination of nitroimidazole residues — Liquid chromatography-tandem mass spectrometry method

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 17
Publication date:: June 30, 2026
Edition:: ISO IS 23822 edition 1 version 1
ICS:: 67.120.20

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Description (English):

This document specifies a liquid chromatography tandem mass spectrometry method (LC-MS/MS) for the determination of nitroimidazole residues in eggs and egg products. This document is applicable to eggs and egg products, including whole eggs (fresh, frozen or pasteurized), egg yolk (liquid, frozen, dried or pasteurized), egg white (liquid, frozen, dried or pasteurized), whole egg powder, egg white powder, egg yolk powder, and liquid or dried albumin preparations.

Technical details

Technical committee: ISO/TC 34/SC 6 - Meat, poultry, fish, eggs and their products
SKU: ISO 23822:2026

Abstract

Overview

ISO 23822:2026 – Eggs and egg products – Determination of nitroimidazole residues - Liquid chromatography-tandem mass spectrometry method sets out an internationally harmonized procedure for quantifying nitroimidazole residues in eggs and egg-derived products using LC-MS/MS (liquid chromatography-tandem mass spectrometry). Developed under ISO/TC 34/SC 6, this standard ensures food safety compliance and supports quality assurance for eggs and egg products (including fresh, frozen, pasteurized, and powdered formats). Implementing ISO 23822:2026 enables laboratories and regulatory authorities to reliably detect and confirm the presence of nitroimidazole residues, ensuring that products meet legal and safety requirements.

Key Topics

Nitroimidazole Residues: This standard covers the determination of several nitroimidazole compounds, including metronidazole, dimetridazole, ronidazole, ipronidazole, and their hydroxy derivatives-substances of concern due to their banned or limited use in food-producing animals.
Applicability: Applicable to a wide range of egg and egg product matrices:
- Whole eggs (fresh, frozen, pasteurized)
- Egg yolk and white (liquid, frozen, dried, pasteurized)
- Egg powders (whole, yolk, white)
- Albumin preparations
Sample Preparation: Detailed protocols for homogenizing, reconstituting, and storing test samples to minimize alterations and maintain integrity.
Analytical Method: Outlines the full LC-MS/MS workflow, including:
- Extraction using acetonitrile
- Purification with solid-phase extraction cartridges
- Analytical separation via HPLC and detection by mass spectrometry
- Use of isotope-labelled internal standards for quantification
Validation and Performance: Specifies method sensitivity with defined limits of detection (0.4 µg/kg) and quantification (1.0 µg/kg), and includes collaborative data on precision (coefficient of variation

Related standards

Other ISO standards

Overview

Key Topics

Nitroimidazole Residues: This standard covers the determination of several nitroimidazole compounds, including metronidazole, dimetridazole, ronidazole, ipronidazole, and their hydroxy derivatives-substances of concern due to their banned or limited use in food-producing animals.
Applicability: Applicable to a wide range of egg and egg product matrices:
- Whole eggs (fresh, frozen, pasteurized)
- Egg yolk and white (liquid, frozen, dried, pasteurized)
- Egg powders (whole, yolk, white)
- Albumin preparations
Sample Preparation: Detailed protocols for homogenizing, reconstituting, and storing test samples to minimize alterations and maintain integrity.
Analytical Method: Outlines the full LC-MS/MS workflow, including:
- Extraction using acetonitrile
- Purification with solid-phase extraction cartridges
- Analytical separation via HPLC and detection by mass spectrometry
- Use of isotope-labelled internal standards for quantification
Validation and Performance: Specifies method sensitivity with defined limits of detection (0.4 µg/kg) and quantification (1.0 µg/kg), and includes collaborative data on precision (coefficient of variation

ISO 23822:2026 PDF

Technical details

Abstract

Overview

Key Topics

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Overview

Key Topics

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