ISO 3356:2009 PDF

Milk — Determination of alkaline phosphatase

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Title (English):

ISO 3356:2009

Milk — Determination of alkaline phosphatase

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 12
Publication date:: March 2, 2009
Edition:: ISO IS 3356 edition 2 version 1
ICS:: 67.100.10

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Description (English):

ISO 3356|IDF 63:2009 specifies a method for the determination of alkaline phosphatase activity in milk. The method applies to alkaline phosphatase activities not less than 1 µg of phenol per millilitre. The method is also suitable for the determination of alkaline phosphatase activity in milk powder, buttermilk and buttermilk powder, whey and whey powder.

Technical details

Technical committee: ISO/TC 34/SC 5 - Milk and milk products
SKU: ISO 3356:2009

Abstract

Overview

ISO 3356:2009 / IDF 63:2009 specifies a standardized laboratory method for the determination of alkaline phosphatase (ALP) activity in milk. The procedure quantifies ALP activity as the mass of phenol liberated (µg phenol per ml) and is applicable to raw and processed milk, milk powder, buttermilk and powders, whey and whey powder. The method covers activities not less than 1 µg phenol/ml and includes sample preparation, reagent recipes, measurement steps and reporting requirements.

Key topics and technical requirements

Analytical principle: ALP in the sample hydrolyzes disodium phenylphosphate at pH 10.6 and 37 °C (1 h), releasing phenol which reacts with 2,6-dibromoquinonechloroimide (Gibbs reagent, BQC) to form a blue dye (dibromoindophenol) measured photometrically at 610 nm.
Reagents and buffers: Detailed formulations provided for barium borate–hydroxide buffer, colour development buffers, disodium phenylphosphate substrate, zinc–copper precipitant, BQC (Gibbs reagent) and phenol standards.
Apparatus: Photometer (610 nm), thermostatted water bath (37 ± 1 °C), boiling water bath, volumetric glassware, pipettes and filtration equipment.
Sample preparation: Homogenize milk samples; reconstitute powders (10 g in 90 ml water, ≤35 °C); neutralize acidic samples (pH

Related standards

Other ISO standards

Overview

Key topics and technical requirements

Analytical principle: ALP in the sample hydrolyzes disodium phenylphosphate at pH 10.6 and 37 °C (1 h), releasing phenol which reacts with 2,6-dibromoquinonechloroimide (Gibbs reagent, BQC) to form a blue dye (dibromoindophenol) measured photometrically at 610 nm.
Reagents and buffers: Detailed formulations provided for barium borate–hydroxide buffer, colour development buffers, disodium phenylphosphate substrate, zinc–copper precipitant, BQC (Gibbs reagent) and phenol standards.
Apparatus: Photometer (610 nm), thermostatted water bath (37 ± 1 °C), boiling water bath, volumetric glassware, pipettes and filtration equipment.
Sample preparation: Homogenize milk samples; reconstitute powders (10 g in 90 ml water, ≤35 °C); neutralize acidic samples (pH

ISO 3356:2009 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

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Abstract

Overview

Key topics and technical requirements

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