ISO 3594:1976 PDF
Milk fat — Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method)
Open preview (PDF)Milk fat — Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method)Milk fat — Detection of vegetable fat by gas-liquid chromatography of sterols (Reference method)
- Document status:
- Withdrawn
- Format:
- Electronic (PDF)
- Number of pages:
- 5
- Publication date:
- December 1, 1976
- Edition:
- ISO IS 3594 edition 1 version 1
- ICS:
- 67.100.10
The method consists in preparing of sterol digitonides and dissolving in a mixture of formamide and dimethylformamide, extraction of the liberated sterols with pentane, separation of the sterols using a suitable gas chromatograph. If, on the chromatogram, a peak with the retention time of beta-sitosterol is obtained, the presence of vegetable fat in the sample is demonstrated. Peaks of other phytosterols may support this conclusion.
Technical details
- Technical committee
- ISO/TC 34/SC 5 - Milk and milk products
- SKU
- ISO 3594:1976


