ISO 6655:1997 PDF

Animal feeding stuffs — Determination of soluble nitrogen content after treatment with pepsin in dilute hydrochloric acid

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Title (English):

ISO 6655:1997

Animal feeding stuffs — Determination of soluble nitrogen content after treatment with pepsin in dilute hydrochloric acid

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 10
Publication date:: August 7, 1997
Edition:: ISO IS 6655 edition 1 version 1
ICS:: 65.120

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Technical details

Technical committee: ISO/TC 34/SC 10 - Animal feeding stuffs
SKU: ISO 6655:1997

Abstract

Overview

ISO 6655:1997 specifies an analytical method for determining the soluble nitrogen content of animal feeding stuffs after treatment with pepsin in dilute hydrochloric acid. The procedure measures nitrogen solubilised by in vitro pepsin digestion and reports results as soluble nitrogen (g/kg) or converted to soluble crude protein (factor 6.25). The standard clarifies that results do not distinguish protein nitrogen from non‑protein nitrogen and are not a direct measure of in vivo digestibility.

Key topics and technical requirements

Principle: Incubate a prepared feed test portion with a pepsin solution in dilute HCl, filter, then determine nitrogen in the filtrate or in the residue by the Kjeldahl method (see ISO 5983).
Reagents: Use analytical‑grade reagents and low‑nitrogen water. Key specifications include dilute HCl c(HCl) = 0.075 mol/L and pepsin with activity 2.0 units/mg; a pepsin working solution of about 400 units/L is prepared immediately before use.
Procedure highlights:
- Test portion ~2 g in 500 mL flask with 450 mL pepsin solution pre‑heated to 40 °C.
- Maintain pH

Related standards

Mentioned in the description and other ISO standards

Overview

Key topics and technical requirements

Principle: Incubate a prepared feed test portion with a pepsin solution in dilute HCl, filter, then determine nitrogen in the filtrate or in the residue by the Kjeldahl method (see ISO 5983).
Reagents: Use analytical‑grade reagents and low‑nitrogen water. Key specifications include dilute HCl c(HCl) = 0.075 mol/L and pepsin with activity 2.0 units/mg; a pepsin working solution of about 400 units/L is prepared immediately before use.
Procedure highlights:
- Test portion ~2 g in 500 mL flask with 450 mL pepsin solution pre‑heated to 40 °C.
- Maintain pH

ISO 6655:1997 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Related standards

Abstract

Overview

Key topics and technical requirements

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