Overview

SIST EN ISO 11701:2010 is an internationally recognized standard developed by CEN and ISO for the quantitative determination of phospholipid content in lecithins derived from vegetable fats and oils. The standard specifies a validated method that uses high performance liquid chromatography (HPLC) with a diol column and a light-scattering detector. This analytical approach ensures accurate identification and quantification of individual phospholipids, supporting quality control and compliance in the food, feed, and lecithin processing industries.

Keywords: vegetable lecithins, phospholipid determination, HPLC, light-scattering detector, vegetable oils, lecithin analysis, standard methods

Key Topics

Scope and Applicability
- Applies to crude, oil-containing lecithins, oil-free lecithins, and lecithin fractions from vegetable sources.
- Not suitable for animal lecithins, ruminant lecithins, or enzymatically hydrolysed lecithins due to insufficient peak separation for certain lysophospholipids.
Principle of Method
- Phospholipids in lecithins are separated on a diol HPLC column.
- Quantification is achieved using a calibrated light-scattering detector and certified reference mixtures.
Test Sample Preparation
- Importance of representative, homogenized samples.
- Samples are dissolved in specific solvent mixtures prior to analysis.
Precision and Repeatability
- Method precision is defined through international interlaboratory tests.
- Parameters for repeatability and reproducibility ensure reliability across laboratories.

Applications

Quality Control in Lecithin Production
- Ensures accurate phospholipid profiling in food-grade and technical-grade lecithins.
- Helps manufacturers verify that lecithin products meet regulatory and customer specifications.
Food and Feed Industry
- Useful for verifying the composition of lecithins used as emulsifiers in processed foods.
- Enables producers to standardize product functionality and performance.
Research and Development
- Supports technical research in the development of new lecithin fractions or applications.
- Facilitates investigation of phospholipid composition for nutritional labeling or functional food innovation.
Supply Chain Compliance
- Assists in certifying the authenticity and purity of vegetable lecithin fractions supplied to various industries.
- Meets international requirements for consistent product analysis and reporting.

Related Standards

ISO 661: Animal and Vegetable Fats and Oils - Preparation of Test Sample
- Provides guidance on test sample preparation, an essential pre-requisite for reliable HPLC analysis.
ISO 5555: Animal and Vegetable Fats and Oils - Sampling
- Establishes standardized procedures for sample collection, crucial for representative testing.
ISO 5725 Series: Accuracy (Trueness and Precision) of Measurement Methods and Results
- Defines methodology for assessing repeatability and reproducibility of analytical methods.
ISO 1042: Laboratory Glassware - One-mark Volumetric Flasks
- Specifies requirements for volumetric equipment used in sample and standard solution preparation.

Practical Value

Adhering to SIST EN ISO 11701:2010 provides the following practical advantages:

Assures Global Acceptance: Use of a harmonized, internationally recognized test provides credibility in global supply chains.
Supports Regulatory Compliance: Provides a scientific basis for compliance with food safety and ingredients regulations.
Enhances Product Consistency: Detailed phospholipid profiling helps maintain product quality and consistency, key for industrial applications.
Reduces Analytical Uncertainty: Standardized methodology minimizes variability and sources of error in phospholipid determination.

By implementing SIST EN ISO 11701:2010, laboratories and industry stakeholders ensure that the determination of phospholipids in vegetable lecithins is accurate, repeatable, and compliant with international best practices.

Overview

Keywords: vegetable lecithins, phospholipid determination, HPLC, light-scattering detector, vegetable oils, lecithin analysis, standard methods

Key Topics

Scope and Applicability
- Applies to crude, oil-containing lecithins, oil-free lecithins, and lecithin fractions from vegetable sources.
- Not suitable for animal lecithins, ruminant lecithins, or enzymatically hydrolysed lecithins due to insufficient peak separation for certain lysophospholipids.
Principle of Method
- Phospholipids in lecithins are separated on a diol HPLC column.
- Quantification is achieved using a calibrated light-scattering detector and certified reference mixtures.
Test Sample Preparation
- Importance of representative, homogenized samples.
- Samples are dissolved in specific solvent mixtures prior to analysis.
Precision and Repeatability
- Method precision is defined through international interlaboratory tests.
- Parameters for repeatability and reproducibility ensure reliability across laboratories.

Applications

Quality Control in Lecithin Production
- Ensures accurate phospholipid profiling in food-grade and technical-grade lecithins.
- Helps manufacturers verify that lecithin products meet regulatory and customer specifications.
Food and Feed Industry
- Useful for verifying the composition of lecithins used as emulsifiers in processed foods.
- Enables producers to standardize product functionality and performance.
Research and Development
- Supports technical research in the development of new lecithin fractions or applications.
- Facilitates investigation of phospholipid composition for nutritional labeling or functional food innovation.
Supply Chain Compliance
- Assists in certifying the authenticity and purity of vegetable lecithin fractions supplied to various industries.
- Meets international requirements for consistent product analysis and reporting.

Related Standards

ISO 661: Animal and Vegetable Fats and Oils - Preparation of Test Sample
- Provides guidance on test sample preparation, an essential pre-requisite for reliable HPLC analysis.
ISO 5555: Animal and Vegetable Fats and Oils - Sampling
- Establishes standardized procedures for sample collection, crucial for representative testing.
ISO 5725 Series: Accuracy (Trueness and Precision) of Measurement Methods and Results
- Defines methodology for assessing repeatability and reproducibility of analytical methods.
ISO 1042: Laboratory Glassware - One-mark Volumetric Flasks
- Specifies requirements for volumetric equipment used in sample and standard solution preparation.

Practical Value

Adhering to SIST EN ISO 11701:2010 provides the following practical advantages:

Assures Global Acceptance: Use of a harmonized, internationally recognized test provides credibility in global supply chains.
Supports Regulatory Compliance: Provides a scientific basis for compliance with food safety and ingredients regulations.
Enhances Product Consistency: Detailed phospholipid profiling helps maintain product quality and consistency, key for industrial applications.
Reduces Analytical Uncertainty: Standardized methodology minimizes variability and sources of error in phospholipid determination.

SIST EN ISO 11701:2010 PDF

Technical details

Abstract

Overview

Key Topics

Applications

Related Standards

Practical Value

Related standards

Abstract

Overview

Key Topics

Applications

Related Standards

Practical Value

Related standards

Animal and vegetable fats and oils. Preparation of test sample

Animal and vegetable fats and oils - Sampling - Amendment 1: Flexitanks (ISO 5555:2001/A1:2014)

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