Overview
EN ISO 24199:2022 (ISO 24199:2022) specifies an analytical gas chromatographic method for the determination of nicotine in vapour product emissions. The standard describes how to generate and collect aerosol from vapour products (e‑cigarettes, vaping devices) and quantitate nicotine in the collected emissions using capillary gas chromatography with flame ionization detection (GC‑FID). It is intended to provide reliable, comparable nicotine emission data for testing laboratories, manufacturers and regulators.
Key topics and technical requirements
- Scope and principle: Quantification of nicotine in aerosol collected from vapour products; results expressed per puff, per aerosol collected mass (ACM) or per puff block.
- Aerosol generation & sample collection: Emissions shall be generated and collected in accordance with routine analytical vaping machine procedures (see ISO 20768 referenced). Aerosol is collected on glass fibre filter pads and ACM is determined gravimetrically.
- Sample extraction: Collected material is extracted with isopropanol containing an internal standard. Typical internal standards include quinaldine or n‑heptadecane; alternatives (e.g., n‑octadecane) require validation to avoid co‑elution.
- Chromatography & detection: Analysis by capillary GC‑FID with internal standard calibration; calibration requires a series of at least five calibration solutions prepared from a nicotine reference substance of known purity.
- Reagents & gases: High‑purity carrier gas (He, N2 or H2) and auxiliary gases for FID; isopropanol extraction solution; certified nicotine reference material (≥98 % purity recommended).
- Quality and validation: Sections on repeatability and reproducibility, interlaboratory study results (based on CORESTA CRM 84 origins), calibration, chromatogram review (Annex A example) and comprehensive test reporting requirements.
Practical applications
- Regulatory compliance and reporting of nicotine emissions from vapour products.
- Routine quality control and batch testing in manufacturing.
- Research and product development for formulation or device design changes.
- Interlaboratory studies and method validation to support harmonized measurement across labs.
Who should use this standard
- Analytical testing laboratories performing nicotine determination in vaping emissions.
- Vapour product manufacturers and R&D teams.
- Regulatory bodies and conformity assessment organizations requiring standardized emission data.
- Academic and contract research organizations studying inhalation exposure from electronic nicotine delivery systems.
Related standards and references
- ISO 20768 - routine analytical vaping machine (sample generation).
- ISO 24197 - determination of e‑liquid vaporized mass / aerosol collected mass (under preparation).
- ISO 13276 - verification of nicotine purity.
- CORESTA CRM 84 - methodological basis and interlaboratory study history.
Keywords: EN ISO 24199, ISO 24199:2022, vapour products, nicotine determination, gas chromatographic method, GC‑FID, aerosol collected mass, vaping emissions analysis.
