EN ISO 15303:2008 PDF

Animal and vegetable fats and oils - Detection and identification of a volatile organic contaminant by GC/MS (ISO 15303:2001)

Title (English):

EN ISO 15303:2008

Animal and vegetable fats and oils - Detection and identification of a volatile organic contaminant by GC/MS (ISO 15303:2001)

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 17
Publication date:: November 12, 2008
Edition:: CEN EN 15303 edition 1 version 1
ICS:: 67.200.10

Document language:

enru

Delivery time:1–7 days

Price:66 €

Description (English):

This International Standard specifies a method for the detection and identification of a volatile organic contaminant in edible oils. It is applicable to the identification of volatile industrial chemicals in both crude and refined edible oils that are suspected of being contaminated. It also enables determination of the concentration of the contaminant. This International Standard is not applicable to the determination of the concentration of chemicals that may react with the edible oil or with one of its natural components. In these cases, the presence of the contaminant may sometimes be established on a qualitative basis. Also, this International Standard is not applicable to non-volatile chemicals. This method has been shown to be applicable for the identification of the following compound classes: _ saturated halogenated hydrocarbons; _ unsaturated halogenated hydrocarbons; _ esters; aldehydes; alcohols; amines; ketones; ethers; _ cyclic and aromatic compounds; _ nitrogen compounds; _ acrylates; etc. The method has been evaluated for concentrations in the range of 1 mg/kg to 10 mg/kg.

Technical details

Technical committee: CEN/TC 307 - Oilseeds, vegetables and animal fats and oils and their by-products - Methods of sampling and analys
SKU: EN ISO 15303:2008

Abstract

Overview

EN ISO 15303:2008 (ISO 15303:2001) specifies a standardized GC/MS method for the detection, identification and quantification of volatile organic contaminants in edible oils (animal and vegetable fats and oils). The procedure uses thermal desorption cold-trapping and deuterated internal standards to identify volatile industrial chemicals in both crude and refined oils, with an evaluated quantification range of 1 mg/kg to 10 mg/kg. The standard is intended for suspected contamination incidents and routine laboratory screening.

Key Topics and Technical Requirements

Scope and applicability
- Targets volatile industrial chemicals in edible oils; not applicable to non-volatile compounds or chemicals that react with oil constituents (in such cases only qualitative presence may sometimes be established).
Sampling and sample prep
- Typical analytical sample: 1.00 g oil with 5.0 µL deuterated internal standard (e.g., fully deuterated benzene, ethyl benzene or naphthalene).
- Use a blank carrier oil (RBD groundnut or similar) free of related industrial chemicals.
Analytical principle
- Volatiles are released from an oil aliquot in a thermal desorption cold-trapping (TCT) inlet, trapped below –40 °C, then flash-heated (to 160 °C) and swept into a GC/MS.
GC/MS and column parameters
- Example capillary column: 50 m, methyl polysiloxane (OV101 or equivalent), 0.5 µm film, 0.32 mm ID.
- GC program example: 50 °C (5 min), ramp 7.5 °C/min to 250 °C (hold 5 min).
- MS settings cited: 70 eV ionization, ~1 scan/s, source ~200 °C.
Identification & quantification
- Identification requires matching GC retention time and mass spectrum with a reference standard.
- Quantification uses reconstructed ion chromatograms and response ratios against deuterated internal standards to reduce matrix interference.
Validated compound classes
- Saturated/unsaturated halogenated hydrocarbons, esters, aldehydes, alcohols, amines, ketones, ethers, cyclic/aromatic and nitrogen compounds, acrylates, etc.

Applications and Users

Food testing laboratories verifying oil safety and quality
Vegetable/animal oil processors performing contamination screening
Regulatory agencies and food safety authorities investigating contamination events
Forensic and environmental labs assessing accidental or industrial pollution in edible oils

Related Standards

Normative reference: ISO 661:1989 (Preparation of test sample)
Adopted as: EN ISO 15303:2008 by CEN (European implementation of ISO 15303:2001)

Keywords: EN ISO 15303:2008, ISO 15303:2001, GC/MS, thermal desorption cold-trapping, volatile organic contaminant, edible oils, detection and identification, deuterated internal standard, quantification 1–10 mg/kg.

Related standards

Mentioned in the description and other EN standards

Abstract

Overview

Key Topics and Technical Requirements

Scope and applicability
- Targets volatile industrial chemicals in edible oils; not applicable to non-volatile compounds or chemicals that react with oil constituents (in such cases only qualitative presence may sometimes be established).
Sampling and sample prep
- Typical analytical sample: 1.00 g oil with 5.0 µL deuterated internal standard (e.g., fully deuterated benzene, ethyl benzene or naphthalene).
- Use a blank carrier oil (RBD groundnut or similar) free of related industrial chemicals.
Analytical principle
- Volatiles are released from an oil aliquot in a thermal desorption cold-trapping (TCT) inlet, trapped below –40 °C, then flash-heated (to 160 °C) and swept into a GC/MS.
GC/MS and column parameters
- Example capillary column: 50 m, methyl polysiloxane (OV101 or equivalent), 0.5 µm film, 0.32 mm ID.
- GC program example: 50 °C (5 min), ramp 7.5 °C/min to 250 °C (hold 5 min).
- MS settings cited: 70 eV ionization, ~1 scan/s, source ~200 °C.
Identification & quantification
- Identification requires matching GC retention time and mass spectrum with a reference standard.
- Quantification uses reconstructed ion chromatograms and response ratios against deuterated internal standards to reduce matrix interference.
Validated compound classes
- Saturated/unsaturated halogenated hydrocarbons, esters, aldehydes, alcohols, amines, ketones, ethers, cyclic/aromatic and nitrogen compounds, acrylates, etc.

Applications and Users

Food testing laboratories verifying oil safety and quality
Vegetable/animal oil processors performing contamination screening
Regulatory agencies and food safety authorities investigating contamination events
Forensic and environmental labs assessing accidental or industrial pollution in edible oils

Related Standards

Normative reference: ISO 661:1989 (Preparation of test sample)
Adopted as: EN ISO 15303:2008 by CEN (European implementation of ISO 15303:2001)

EN ISO 15303:2008 PDF

Technical details

Abstract

Overview

Key Topics and Technical Requirements

Applications and Users

Related Standards

Related standards

Abstract

Overview

Key Topics and Technical Requirements

Applications and Users

Related Standards

Related standards

Animal and vegetable fats and oils — Detection and identification of a volatile organic contaminant by GC/MS

Animal and vegetable fats and oils — Preparation of test sample

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