ISO 15303:2001 PDF

Animal and vegetable fats and oils — Detection and identification of a volatile organic contaminant by GC/MS

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Title (English):

ISO 15303:2001

Animal and vegetable fats and oils — Detection and identification of a volatile organic contaminant by GC/MS

Document status:: Active
Format:: Electronic (PDF)
Number of pages:: 10
Publication date:: September 20, 2001
Edition:: ISO IS 15303 edition 1 version 1
ICS:: 67.200.10

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Description (English):

This International Standard specifies a method for the detection and identification of a volatile organic contaminant in edible oils. It is applicable to the identification of volatile industrial chemicals in both crude and refined edible oils that are suspected of being contaminated. It also enables determination of the concentration of the contaminant. This International Standard is not applicable to the determination of the concentration of chemicals that may react with the edible oil or with one of its natural components. In these cases, the presence of the contaminant may sometimes be established on a qualitative basis. Also, this International Standard is not applicable to non-volatile chemicals. This method has been shown to be applicable for the identification of the following compound classes: _ saturated halogenated hydrocarbons; _ unsaturated halogenated hydrocarbons; _ esters; aldehydes; alcohols; amines; ketones; ethers; _ cyclic and aromatic compounds; _ nitrogen compounds; _ acrylates; etc. The method has been evaluated for concentrations in the range of 1 mg/kg to 10 mg/kg.

Technical details

Technical committee: ISO/TC 34/SC 11 - Animal and vegetable fats and oils
SKU: ISO 15303:2001

Abstract

Overview

ISO 15303:2001 - Animal and vegetable fats and oils - Detection and identification of a volatile organic contaminant by GC/MS - specifies a standardized laboratory method to detect, identify and quantify volatile organic contaminants in edible oils. The method uses thermal desorption cold‑trapping combined with gas chromatography–mass spectrometry (GC/MS) and is validated for concentrations in the range 1 mg/kg to 10 mg/kg. It applies to both crude and refined edible oils but excludes non‑volatile contaminants and chemicals that chemically react with the oil.

Key topics and technical requirements

Principle: volatiles evaporate from an oil sample into a cooled trap, then are flash‑released to a GC/MS for separation and spectral identification.
Sample preparation: typically 1.00 g oil with an internal standard (deuterated benzene, ethylbenzene or naphthalene in methanol). Equilibration (overnight recommended) improves results.
Instrumentation:
- Thermal desorption cold‑trapping (TCT) device (trap held below −40 °C, then flash‑heated to 160 °C).
- Capillary GC column: 50 m, methyl polysiloxane (OV101 or equivalent), 0.5 µm film, 0.32 mm i.d.
- GC program: start 50 °C (5 min), ramp 7.5 °C/min to 250 °C (hold 5 min).
- MS conditions: electron impact 70 eV, scan ≥1 scan/s.
Quantification: use deuterated internal standard and reconstructed ion chromatograms; compute concentration from response ratios (R = analyte area / internal standard area).
Identification: requires matching both GC retention time and mass spectrum against an authentic standard.
Applicable compound classes: saturated/unsaturated halogenated hydrocarbons, esters, aldehydes, alcohols, amines, ketones, ethers, cyclic/aromatic and nitrogen compounds, acrylates, etc.
Performance/precision: repeatability limit ~0.6 mg/kg (5% worst case); reproducibility limit ~1.43 mg/kg.

Practical applications and users

Use cases:
- Investigation of suspected industrial contamination of edible oils (crude or refined).
- Regulatory compliance testing and food safety incident response.
- Quality control in oil refining, storage, transport and cargo inspection.
Typical users:
- Food testing laboratories and analytical chemists specializing in oils and fats.
- Regulatory agencies, port authorities and quality assurance teams in the edible oil supply chain.
- CROs and research labs performing GC/MS contaminant analysis.

Related standards

Normative reference: ISO 661:1989 (preparation of test sample for fats and oils).
Precision assessment references cited: ISO 5725 series (interlaboratory precision guidance).

Keywords: ISO 15303:2001, GC/MS, volatile organic contaminant, edible oils, thermal desorption cold‑trapping, internal standard, quantitative detection 1–10 mg/kg.

Related standards

Mentioned in the description and other ISO standards

Abstract

Overview

Key topics and technical requirements

Principle: volatiles evaporate from an oil sample into a cooled trap, then are flash‑released to a GC/MS for separation and spectral identification.
Sample preparation: typically 1.00 g oil with an internal standard (deuterated benzene, ethylbenzene or naphthalene in methanol). Equilibration (overnight recommended) improves results.
Instrumentation:
- Thermal desorption cold‑trapping (TCT) device (trap held below −40 °C, then flash‑heated to 160 °C).
- Capillary GC column: 50 m, methyl polysiloxane (OV101 or equivalent), 0.5 µm film, 0.32 mm i.d.
- GC program: start 50 °C (5 min), ramp 7.5 °C/min to 250 °C (hold 5 min).
- MS conditions: electron impact 70 eV, scan ≥1 scan/s.
Quantification: use deuterated internal standard and reconstructed ion chromatograms; compute concentration from response ratios (R = analyte area / internal standard area).
Identification: requires matching both GC retention time and mass spectrum against an authentic standard.
Applicable compound classes: saturated/unsaturated halogenated hydrocarbons, esters, aldehydes, alcohols, amines, ketones, ethers, cyclic/aromatic and nitrogen compounds, acrylates, etc.
Performance/precision: repeatability limit ~0.6 mg/kg (5% worst case); reproducibility limit ~1.43 mg/kg.

Practical applications and users

Use cases:
- Investigation of suspected industrial contamination of edible oils (crude or refined).
- Regulatory compliance testing and food safety incident response.
- Quality control in oil refining, storage, transport and cargo inspection.
Typical users:
- Food testing laboratories and analytical chemists specializing in oils and fats.
- Regulatory agencies, port authorities and quality assurance teams in the edible oil supply chain.
- CROs and research labs performing GC/MS contaminant analysis.

Related standards

Normative reference: ISO 661:1989 (preparation of test sample for fats and oils).
Precision assessment references cited: ISO 5725 series (interlaboratory precision guidance).

Keywords: ISO 15303:2001, GC/MS, volatile organic contaminant, edible oils, thermal desorption cold‑trapping, internal standard, quantitative detection 1–10 mg/kg.

ISO 15303:2001 PDF

Technical details

Abstract

Overview

Key topics and technical requirements

Practical applications and users

Related standards

Related standards

Abstract

Overview

Key topics and technical requirements

Practical applications and users

Related standards

Related standards

Animal and vegetable fats and oils — Preparation of test sample

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